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標題: Detection of allergies using a silver nanoparticle modified nanostructured biosensor
作者: Liu, Yi-Fen
Tsai, Jaw-Ji
Chin, Yu-Ting
Liao, En-Chih
Wu, Chia-Che
Wang, Gou-Jen
關鍵字: Allergy detection Nanostructured biosensor;Gold nanoparticles;Silver nanoparticles;Electrochemical impedance spectroscopy analysis
Project: Sensors and Actuators B: Chemical,Volume 171– 172, page(s) 1095– 1100.
The IgE (Immunoglobulin E) in the serum of an asthma patient is a useful index for the detection of
allergy diseases. In this study, a nanostructured biosensor with uniformly deposited gold nanoparticles
(GNPs) is used as the sensing electrode for fast and low serum consuming detection of the IgE in an
allergy patient’s serum. To enhance the charge transfer efficiency of the biosensor, silver nanoparticles
(SNPs) were deposited on the GNP layer. The group 2 allergen, Der p2, was used to indicate IgE. To
ensure the specificity of detection, affinity purified goat anti-human IgE antibody was further immobilized
to the IgE. After immobilizing the anti-IgE, electrochemical impedance spectroscopy (EIS) analysis was
implemented to examine the concentration of the target IgE which was displayed in terms of a Nyquist
plot. Blood serum samples with known allergy levels detected by the commercially available ImmunoCAP
were used for verification of the sensor results. It is observed that the difference in the charge transfer
resistance ( Ret) between the Der p2 immobilized electrode and the anti-IgE bonded electrode for each
individual serum sample closely correlates to its ImmunoCAP class. The blood serum detection results
indicate that the presented nanostructured biosensor is able to detect a patient’s allergy level with low
sample consumption, short sample preparation time, and quick processing.
ISSN: 0925-4005
DOI: 10.1016/j.snb.2012.06.039
Appears in Collections:機械工程學系所

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