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|標題:||Expression of 25 high egg production related transcripts that identified from hypothalamus and pituitary gland in red-feather Taiwan country chickens||作者:||Chen, L.R.
|關鍵字:||chicken;high egg production;hypothalamus;pituitary gland;transcripts;quantitative RT-PCR;hen gallus-domesticus;luteinizing-hormone;ovulatory cycle;prolactin;progesterone;performance;secretion;proteins;database;release||Project:||Animal Reproduction Science||期刊/報告no：:||Animal Reproduction Science, Volume 100, Issue 1-2, Page(s) 172-185.||摘要:||
Expression levels of 33 high egg production candidate transcripts in Red-feather Taiwan country chickens (TCCs) were examined by quantitative reverse-transcription (RT) polymerase chain reactions (PCR) in this study. Candidate transcripts were previously identified from a L2-B (L2-subtract-B) hypothalamus/pituitary gland subtractive cDNA library. In this subtractive cDNA library, two divergently selected strains of TCCs, B and L2 were used. These two strains were originated from one single population and were further subjected (since 1982) to the selections of body weight/comb size (B) and eggs to 40 wk of age (L2), respectively. Hypothalamuses and pituitary glands that sampled from Red-feather TCCs were previously grouped into high (Red-high; n = 20) and low (Red-low-, it = 20) egg productions based on the rate of lay after 1st egg (hen-day laying rate; %). Rates of lay after 1st egg (mean +/- S.E.) in the Red-high and the Red-low sub-populations were 72.2 +/- 0.6 and 23.0 +/- 3.5, respectively (P < 0.01). Quantitative RT-PCR validated that 25 candidate transcripts were significantly higher expressed in the Red-high than in the Red-low hens. These transcripts were ANP32A, BDH, CDC42, CNTN1, COMT, CPE, CTNNB1, DIO2, EIF4E, GARNL1, HSPCA, LAPTM4B, MBP, NAPIL4, NCAM1, PARK7, PCDHA@, PGDS, PLAG1, PRL, RAD21, SARIA, SCG2, STMN1 and UFM1. Among these transcripts, 15 (79.0%), 13 (68.4%), and 12 (63.2%) genes were annotated to involve in cellular physiological process (GO:0050875), metabolism (GO:0008152) and cell communication (GO:0007154). Identified transcripts that related to high egg production are most active in focal adhesion, adherens junction, MAPK signaling, tight junction and cell adhesion pathways. (C) 2006 Elsevier B.V. All rights reserved.
|Appears in Collections:||動物科學系|
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