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|標題:||Association of heat shock protein 70 with semen quality in boars||作者:||Huang, S.Y.
|關鍵字:||heat shock protein 70;semen quality;season;boar;elevated ambient-temperature;substantial decrease;spermatogenic cells;stress-response;thermometer;hsp104;hsp70;sperm||Project:||Animal Reproduction Science||期刊/報告no：:||Animal Reproduction Science, Volume 63, Issue 3-4, Page(s) 231-240.||摘要:||
This study attempted to clarify the relationship between the levels of 70 kDa heat shock protein (HSP70) and semen quality in boars. Semen samples from 29 (13 Duroc, 9 Landrace, and 7 Yorkshire) boars (mean age=25.2+/-2.2 months) were examined. Three to four ejaculates per boar, collected during cool and hot seasons, were evaluated in terms of the sperm concentration, sperm motility, percentage of normal and abnormal sperm, as well as percentage of sperm with proximal and distal plasma droplets. Significant seasonal and breed differences in semen quality were observed. Experimental results indicate that the semen quality of Landrace boars was better than those of Yorkshire and Duroc boars (P<0.05) and semen quality declined significantly during the hot season (P<0.05). One-dimensional SDS-PAGE analysis of spermatozoa proteins indicated that protein profiles did not significantly differ between seasons and among breeds. Both constitutive and stress-inducible form of HSP70 were detected in boar spermatozoa by Western blot analysis. The level of HSP70, which revealed no difference among breeds within a season, was significantly lower during the hot season in all the three breeds (P<0.05). although there appeared to be low correlation coefficients between the level of HSP70 and semen quality traits, the semen quality tended to decline significantly in samples with a lower level of HSP70. Results in this study suggest that the levels of HSP70 in boar spermatozoa are significantly lower during the hot season and might be associated with semen quality. (C) 2000 Elsevier Science B.V. All rights reserved.
|Appears in Collections:||動物科學系|
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