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標題: A reference map and identification of porcine testis proteins using 2-DE and MS
作者: Huang, S.Y.
Lin, J.H.
Chen, Y.H.
Chuang, C.K.
Lin, E.C.
Huang, M.C.
Sun, H.F.S.
Lee, W.C.
關鍵字: 2-DE;MALDI-MS;porcine testis;protein database;proteome;glutathione-s-transferase;mouse spermatogenic cells;rat testis;2-dimensional electrophoresis;postnatal-development;carbonyl;reductase;mass-spectrometry;round spermatids;expression;spermatocytes
Project: Proteomics
期刊/報告no:: Proteomics, Volume 5, Issue 16, Page(s) 4205-4212.
The development of the testis is essential for maturation of male mammals. A complete understanding of proteins expressed in the testis will provide biological information on many reproductive dysfunctions in males. The purposes of this study were to apply a proteomic approach to investigating protein composition and to establish a 2-D PAGE reference map for porcine testis proteins. MALDI-TOF M S was performed for protein identification. When 1 mg of total proteins was assayed by 2-D PAGE and stained with colloidal CBB, more than 400 proteins with a pI of pH 3-10 and M-r of 10-200 kDa could be detected. Protein expression varied among individuals, with CV between 4.7 and 131.5%. A total of 447 protein spots were excised for identification, among which 337 spots were identified by searching the mass spectra against the NCBInr database. Identification of the remaining 110 spots was unsuccessful. A 2-D PAGE-based porcine testis protein database has been constructed on the basis of the results and will be published on the WWW. This database should be valuable for investigating the developmental biology and pathology of porcine testis.
ISSN: 1615-9853
DOI: 10.1002/pmic.200401284
Appears in Collections:動物科學系

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