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標題: Female-specific DNA sequences in geese
作者: Huang, M.C.
Lin, W.C.
Horng, Y.M.
Rouvier, R.
Huang, C.W.
關鍵字: h-y-antigen;sex-ratio;embryos;identification;blastocysts;birds
Project: British Poultry Science
期刊/報告no:: British Poultry Science, Volume 44, Issue 3, Page(s) 359-364.
1. The OPAE random primers (Operon Technologies, Inc., CA) were used for random amplified polymorphic DNA (RAPD) fingerprinting in Chinese, White Roman and Landaise geese. One of these primers, OPAE-06, produced a 938-bp sex-specific fragment in all females and in no males of Chinese geese only. 2. A novel female-specific DNA sequence in Chinese goose was cloned and sequenced. Two primers, CGSex-F and CGSex-R, were designed in order to amplify a 912-bp sex-specific polymerase chain reaction (PCR) fragment on genomic DNA from female geese. 3. It was shown that a simple and effective PCR-based sexing technique could be used in the three goose breeds studied. 4. Nucleotide sequencing of the sex-specific fragments in White Roman and Landaise geese was performed and sequence differences were observed among these three breeds.
ISSN: 0007-1668
DOI: 10.1080/0007166031000085562
Appears in Collections:動物科學系

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