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|標題:||Female-specific DNA sequences in geese||作者:||Huang, M.C.
|關鍵字:||h-y-antigen;sex-ratio;embryos;identification;blastocysts;birds||Project:||British Poultry Science||期刊/報告no：:||British Poultry Science, Volume 44, Issue 3, Page(s) 359-364.||摘要:||
1. The OPAE random primers (Operon Technologies, Inc., CA) were used for random amplified polymorphic DNA (RAPD) fingerprinting in Chinese, White Roman and Landaise geese. One of these primers, OPAE-06, produced a 938-bp sex-specific fragment in all females and in no males of Chinese geese only. 2. A novel female-specific DNA sequence in Chinese goose was cloned and sequenced. Two primers, CGSex-F and CGSex-R, were designed in order to amplify a 912-bp sex-specific polymerase chain reaction (PCR) fragment on genomic DNA from female geese. 3. It was shown that a simple and effective PCR-based sexing technique could be used in the three goose breeds studied. 4. Nucleotide sequencing of the sex-specific fragments in White Roman and Landaise geese was performed and sequence differences were observed among these three breeds.
|Appears in Collections:||動物科學系|
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