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http://hdl.handle.net/11455/45167
標題: | SSCP analysis in the D-loop region of porcine mitochondrial DNA as confirmed by sequence diversity | 作者: | Lin, C.S. 黃木秋 Liu, C.Y. Wu, H.T. Sun, Y.L. Chang, L.C. Yen, N.T. Yang, P.C. Huang, M.C. Mao, S.J.T. |
關鍵字: | cytoplasmic genetic-variation;rapid evolution;pcr-sscp;polymorphism;mutations;origin;breeds;pigs | Project: | Journal of Animal Breeding and Genetics-Zeitschrift Fur Tierzuchtung Und Zuchtungsbiologie | 期刊/報告no:: | Journal of Animal Breeding and Genetics-Zeitschrift Fur Tierzuchtung Und Zuchtungsbiologie, Volume 115, Issue 1, Page(s) 73-78. | 摘要: | Sequence polymorphism of the displacement loop (D-loop) region in mitochondrial DNA (mtDNA) is a cytoplasmic DNA marker. Since the analysis of single-strand conformation polymorphism (SSCP) has been used for detection of substitutions in nucleotide sequence, this technique was employed to investigate the porcine sequence polymorphism in the D-loop region. The primers were prepared to amplify the most polymorphic site with 392-bp in the D-loop region from whole blood of pigs. The PCR-SSCP patterns were Identical in European breeds Landrace and Duroc, bur were different in Yorkshire and in Taiwanese breeds Lanyu and Taoyuan. Nucleotide sequence analysis in the 392-bp PCR products were completely in accordance with the PCR-SSCP patterns. There was only a single nucleotide substitution between Yorkshire and Landrace/Duroc pig and 2-3 nucleotide substitutions (dependent on the breed and individual) between Taiwanese Lanyu and Taoyuan. As many as 10-11 nucleotides were different between European and Taiwanese breeds in which most differences clustered Fig. 3. Nucleotide sequence surrounding the highly variable D-loop region with comparison between the commercial European and Taiwanese breeds. The different nucleotide sequences between two groups are marked by underlining. The region compared is the nucleotides ranging from 6 to 46 (shown in parentheses) as shown in Fig. 2 within a certain region in the D-loop. The results suggest that PCR-SSCP analysis may be used for detecting single nucleotide substitution in the porcine mtDNA D-loop region and show the diversified sequence in this region between European and Asian origins. |
URI: | http://hdl.handle.net/11455/45167 | ISSN: | 0931-2668 |
Appears in Collections: | 動物科學系 |
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