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標題: Female-specific DNA sequences in ostriches
作者: Wu, C.P.
Horng, Y.M.
Yang, K.T.
Huang, C.W.
Huang, M.C.
關鍵字: ostrich;genetic marker;sex specific sequence;sexing;non-ratite birds;sex-chromosomes;rapd assay;identification;markers
Project: Molecular and Cellular Probes
期刊/報告no:: Molecular and Cellular Probes, Volume 20, Issue 5, Page(s) 307-310.
Ostrich absence of heteromorphic sex chromosomes, unique sequences or markers located in the ostrich W-chromosome. Random amplified polymorphic DNA (RAPD) fingerprinting was carried out to investigate the sex-specific DNA sequence for sexing in ostrich. One hundred and forty random primers were used for random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). One of these primers, OPAJ-13, produced a sex-specific band only found in tested females, which was isolated and constructed into plasmids for nucleotide sequencing. A 760 bp novel female-specific sequence was obtained. Two primers (OstSexOPAJ13-F and -R) were designed according to the cloned female sequence to amplify the female-specific fragment from genomic DNA of ostriches for sexing by PCR. The sex-specific band was represented in females but none were found in the males. This result showed that the sex of ostrich could be easily and effectively identified using the female-specific primers for PCR technique. (c) 2006 Elsevier Ltd. All rights reserved.
ISSN: 0890-8508
DOI: 10.1016/j.mcp.2006.03.002
Appears in Collections:動物科學系

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