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標題: Effects of glucose concentration on in vitro fertilization in BALB/c mice
作者: Wu, H.T.
Chou, C.K.
Lin, C.S.
Huang, M.C.
關鍵字: mouse sperm;spermatozoa;capacitation;invitro;oocyte;abnormalities;chromosome;morphology;level
Project: Reproduction in Domestic Animals
期刊/報告no:: Reproduction in Domestic Animals, Volume 38, Issue 6, Page(s) 470-474.
BALB/c mice are widely used in genetic, tumour and immunological studies. However, the mice demonstrate a lower reproduction rate, low fertility and small litters, because of their highly genetic homozygoisty. Based on in vitro fertilization (IVF), a routine technique for biomedical studies, it is worth to evaluate the effects to BALB/c mice on IVF efficiency. In order to test the genetic factor affecting the IVF efficiency of BALB/c, four reciprocal IVF tests of BALB/cByJ and FVB/NCrl mice were performed. The results showed that the average fertility of IVF sponsored by FVB/NCrl spermatozoa was 69.6%, but only 12.1% was obtained from BALB/cByJ strain. Effect of glucose contained in the culture medium to the IVF efficiency of BALB/cByJ was also evaluated. The results showed that the fertility of BALB/cByJ spermatozoa incubated with 0, 2.7, 5.5, 11.1 and 22.2 mM of glucose in the TYH medium were 6.8, 9.9, 13.9, 32.7 and 22.2%, respectively. It is showed that IVF efficiency of BALB/cByJ spermatozoa could be improved depending on the concentration of glucose in the IVF medium. According to the results, it is beleived that lower IVF of BALB/cByJ mice might be due to the genetic defect in spermatozoa and increasing glucose in the IVF medium which significantly affect the IVF efficiency of BALB/cByl via activating the spermatozoa.
ISSN: 0936-6768
DOI: 10.1046/j.0936-6768.2003.00465.x
Appears in Collections:動物科學系

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