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標題: Protective immunity conferred by recombinant Pasteurella multocida lipoprotein E (PlpE)
作者: Wu, J.R.
Shien, J.H.
Shieh, H.K.
Chen, C.F.
Chang, P.C.
關鍵字: Lipoprotein E;PlpE;Lipoprotein B;PlpB;Pasteurella multocida;mannheimia-haemolytica serotype-1;membrane protein plpe;cross-protection;fowl cholera;escherichia-coli;vaccine;turkeys;factor(s);sequence
Project: Vaccine
期刊/報告no:: Vaccine, Volume 25, Issue 21, Page(s) 4140-4148.
The genes encoding Pasteurella multocida lipoprotein E (PlpE) and lipoprotein B (PlpB) were cloned from P. multocida strain X-73 (serotype A:1) and expressed in Escherichia coli. The protective immunity conferred by recombinant PlpE (r-PlpE) and PlpB (r-PlpB) on mice and chickens was evaluated. The results showed that mice immunized with 10 mu g of purified r-PlpE were protected (80-100% survival rate) against challenge infection with 10 or 20 LD50 of P. multocida strains X-73 (serotype A:1), P-1059 (serotype A:3) and P-1662 (serotype A:4). In contrast, mice immunized with r-PlpB were not protected. Chickens immunized with 100 mu g of purified r-PlpE were protected (63-100% survival rate) against lethal challenge infection with strains X-73 and P-1662, whereas those immunized with r-PlpB were not. Sequence analyses showed that PlpE from different strains of P. multocida exhibited 90.8-100% sequence identity to each other, suggesting that PIpE might serve as a cross-protective antigen. This is the first report of a recombinant P. multocida antigen that confers cross protection on animals. (c) 2007 Elsevier Ltd. All rights reserved.
ISSN: 0264-410X
DOI: 10.1016/j.vaccine.2007.03.005
Appears in Collections:動物科學系

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