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標題: Quantitative analysis of a high-rate hydrogen-producing microbial community in anaerobic agitated granular sludge bed bioreactors using glucose as substrate
作者: Hung, C.H.
Lee, K.S.
Cheng, L.H.
Huang, Y.H.
Lin, P.J.
Chang, J.S.
關鍵字: fermentative hydrogen production;granular sludge;bioreactor design;bacterial community structure;fluorescence in situ hybridization;targeted oligonucleotide probes;16s ribosomal-rna;biohydrogen;production;activated-sludge;waste;systems;hybridization;populations;kinetics;cultures
Project: Applied Microbiology and Biotechnology
期刊/報告no:: Applied Microbiology and Biotechnology, Volume 75, Issue 3, Page(s) 693-701.
Fermentative H-2 production microbial structure in an agitated granular sludge bed bioreactor was analyzed using fluorescence in situ hybridization (FISH) and polymerase chain reaction-denatured gradient gel electrophoresis (PCR-DGGE). This hydrogen-producing system was operated at four different hydraulic retention times (HRTs) of 4, 2, 1, and 0.5 h and with an influent glucose concentration of 20 g chemical oxygen demand/l. According to the PCR-DGGE analysis, bacterial community structures were mainly composed of Clostridium sp. (possibly Clostridium pasteurianum), Klebsiella oxytoca, and Streptococcus sp. Significant increase of Clostridium/ total cell ratio (68%) was observed when the reactor was operated under higher influent flow rate. The existence of Streptococcus sp. in the reactor became more important when operated under a short HRT as indicated by the ratio of Streptococcus probe-positive cells to Clostridium probe-positive cells changing from 21% (HRT 4 h) to 38% (HRT 0.5 h). FISH images suggested that Streptococcus cells probably acted as seeds for self- flocculated granule formation. Furthermore, combining the inspections with hydrogen production under different HRTs and their corresponding FISH analysis indicated that K oxytoca did not directly contribute to H-2 production but possibly played a role in consuming O-2 to create an anaerobic environment for the hydrogen-producing Clostridium.
ISSN: 0175-7598
DOI: 10.1007/s00253-007-0854-7
Appears in Collections:環境工程學系所

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