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標題: Induction of cysteine proteinase in the encapsulation of Hymenolepis diminuta eggs in the American cockroach, Periplaneta americana
作者: Tu, W.C.
Lai, S.C.
關鍵字: Periplaneta americana;Hymenolepis diminuta;encapsulation;cysteine;proteinase;hemocyte proteinase;insects;larvae;purification;sarcophaga;mechanisms;cestoda;capsule
Project: Journal of Invertebrate Pathology
期刊/報告no:: Journal of Invertebrate Pathology, Volume 92, Issue 2, Page(s) 73-78.
Haemocytes play an essential role in defending invertebrates against pathogens and parasites that enter their haemocoel. In the present study, the cockroach, Periplaneta americana was able to encapsulate Hymenolepis diminuta eggs within 24 h after injection. Proteolytic activity of egg capsules was determined by gelatin zymography. A gelatinase-type proteolytic enzyme with molecular weight about 65 kDa was present at the time of capsule formation. Enzyme activity was obviously inhibited by leupeptin but not by ethylenediamine tetraacetic acid (EDTA) or 1,10-phenanthroline or phenylmethanesulfonyl fluoride (PMSF). Hence, we tentatively characterized this enzyme as a cysteine proteinase. The specificity of the cellular immune response in vivo and the increased cysteine proteinase activity coincided with the capsule size and encapsulation process. The possible function of this cysteine proteinase activity during encapsulation of the H. diminuta eggs by P. americana is discussed. (c) 2006 Elsevier Inc. All rights reserved.
ISSN: 0022-2011
DOI: 10.1016/j.jip.2006.04.002
Appears in Collections:昆蟲學系

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