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標題: Receptor guanylyl cyclases in Inka cells targeted by eclosion hormone
作者: Chang, J.C.
Yang, R.B.
Adams, M.E.
Lu, K.H.
關鍵字: Bactrocera dorsalis;ecdysis;oriental fruit fly;cyclic GMP;ecdysis;triggering hormone;ecdysis-triggering hormone;amino-acid-sequence;manduca-sexta;neuroendocrine control;activating protein;molecular-cloning;endocrine;system;tobacco hornworm;insect ecdysis;nitric-oxide
Project: Proceedings of the National Academy of Sciences of the United States of America
期刊/報告no:: Proceedings of the National Academy of Sciences of the United States of America, Volume 106, Issue 32, Page(s) 13371-13376.
A signature of eclosion hormone (EH) action in insect ecdysis is elevation of cGMP in Inka cells, leading to massive release of ecdysis triggering hormone (ETH) and ecdysis initiation. Although this aspect of EH-induced signal transduction is well known, the receptor mediating this process has not been identified. Here, we describe a receptor guanylyl cyclase BdmGC-1 and its isoform BdmGC-1B in the Oriental fruit fly Bactrocera dorsalis that are activated by EH. The B form exhibits the conserved domains and putative N-glycosylation sites found in BdmGC-1, but possesses an additional 46-amino acid insertion in the extracellular domain and lacks the C-terminal tail of BdmGC-1. Combined immunolabeling and in situ hybridization reveal that BdmGC-1 is expressed in Inka cells. Heterologous expression of BdmGC-1 in HEK cells leads to robust increases in cGMP following exposure to low picomolar concentrations of EH. The B-isoform responds only to higher EH concentrations, suggesting different physiological roles of these cyclases. We propose that BdmGC-1 and BdmGC-1B are high-and low-affinity EH receptors, respectively.
ISSN: 0027-8424
DOI: 10.1073/pnas.0812593106
Appears in Collections:昆蟲學系

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