Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/46025
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dc.contributor.authorChang, J.C.en_US
dc.contributor.author路光暉zh_TW
dc.contributor.authorYang, R.B.en_US
dc.contributor.authorAdams, M.E.en_US
dc.contributor.authorLu, K.H.en_US
dc.date2009zh_TW
dc.date.accessioned2014-06-06T08:16:56Z-
dc.date.available2014-06-06T08:16:56Z-
dc.identifier.issn0027-8424zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/46025-
dc.description.abstractA signature of eclosion hormone (EH) action in insect ecdysis is elevation of cGMP in Inka cells, leading to massive release of ecdysis triggering hormone (ETH) and ecdysis initiation. Although this aspect of EH-induced signal transduction is well known, the receptor mediating this process has not been identified. Here, we describe a receptor guanylyl cyclase BdmGC-1 and its isoform BdmGC-1B in the Oriental fruit fly Bactrocera dorsalis that are activated by EH. The B form exhibits the conserved domains and putative N-glycosylation sites found in BdmGC-1, but possesses an additional 46-amino acid insertion in the extracellular domain and lacks the C-terminal tail of BdmGC-1. Combined immunolabeling and in situ hybridization reveal that BdmGC-1 is expressed in Inka cells. Heterologous expression of BdmGC-1 in HEK cells leads to robust increases in cGMP following exposure to low picomolar concentrations of EH. The B-isoform responds only to higher EH concentrations, suggesting different physiological roles of these cyclases. We propose that BdmGC-1 and BdmGC-1B are high-and low-affinity EH receptors, respectively.en_US
dc.language.isoen_USzh_TW
dc.relationProceedings of the National Academy of Sciences of the United States of Americaen_US
dc.relation.ispartofseriesProceedings of the National Academy of Sciences of the United States of America, Volume 106, Issue 32, Page(s) 13371-13376.en_US
dc.relation.urihttp://dx.doi.org/10.1073/pnas.0812593106en_US
dc.subjectBactrocera dorsalisen_US
dc.subjectecdysisen_US
dc.subjectoriental fruit flyen_US
dc.subjectcyclic GMPen_US
dc.subjectecdysisen_US
dc.subjecttriggering hormoneen_US
dc.subjectecdysis-triggering hormoneen_US
dc.subjectamino-acid-sequenceen_US
dc.subjectmanduca-sextaen_US
dc.subjectneuroendocrine controlen_US
dc.subjectactivating proteinen_US
dc.subjectmolecular-cloningen_US
dc.subjectendocrineen_US
dc.subjectsystemen_US
dc.subjecttobacco hornwormen_US
dc.subjectinsect ecdysisen_US
dc.subjectnitric-oxideen_US
dc.titleReceptor guanylyl cyclases in Inka cells targeted by eclosion hormoneen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1073/pnas.0812593106zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.openairetypeJournal Article-
item.fulltextno fulltext-
Appears in Collections:昆蟲學系
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