Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/46103
標題: cDNA isolation, expression, and hormonal regulation of yolk protein genes in the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)
作者: Chen, Shiu-Ling
Lin, Chung-Pin
Lu, Kuang-Hui
關鍵字: Yolk protein;Fat body;Bactrocera dorsalis;Vitellogenesis;Ecdysteroids;Juvenile hormone
Project: Journal of Insect Physiology, Volume 58, Page(s) 763–770.
摘要: 
Yolk protein (YP) or vitellogenin (Vg), the main component of yolk, is the key nutrient for embryonic
development. YPs, encoded from uncleaved genes existing mainly in cyclorraphan flies, are different from
VGs that are present in most non-cyclorraphan dipterans and other insects. In this study, cDNAs of two
YPs, namely Bdyp1 and Bdyp2 (GenBank accession Nos. AF368053 and AF368054), were isolated in the
oriental fruit fly, Bactrocera dorsalis (Hendel). RT-PCR analysis revealed that Bdyp1 and 2 are expressed
in the fat body and ovary during egg development. However, the expression profiles of Bdyp1 and 2 in
the fat body are different, indicating that divergent mechanisms might exist in the regulation of these
two genes. Twenty-hydroxyecdysone (20E) plays a major role in promoting Bdyp1 expression, yet the
expression of Bdyp2 exhibits a greater response to juvenile hormone (JH) in fat body in vitro. Unexpectedly,
20E-induced expression of both Bdyp1 and 2 is suppressed by JH prior to 20E treatment of in vitro fat
body; conversely, it is enhanced by the addition of JH following 20E treatment.
URI: http://hdl.handle.net/11455/46103
ISSN: 0022-1910
DOI: 10.1016/j.jinsphys.2011.12.007
Appears in Collections:昆蟲學系

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