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標題: In Vitro Regeneration and Genetic Transformation of Cucumis metuliferus through Cotyledon Organogenesis
作者: Lin, Yu-Tsung
Lin, Chia-Wei
Chung, Chien-Hung
Su, Mei-Hsiu
Ho, Hsiu-Yin
Yeh, Shi-Dong
Jan, Fuh-Jyh
Ku, Hsin-Mei
關鍵字: Agrobacterium transformation;beta-glucuronidase;horned melon;watermelon mosaic virus-1;plant-regeneration;melo l;agrobacterium-tumefaciens;somatic embryogenesis;shoot regeneration;mediated transformation;transgenic plants;horned cucumber;resistance
Project: Hortscience, Volume 46, Issue 4, Page(s) 616-621.
This study was undertaken to establish for the first time an efficient regeneration and transformation system for Cucumis metuliferus line PI292190, which is the source of a well-defined resistant gene, Wmv, that provides resistance against Papaya ringspot virus type P (PRSV-P) and PRSV-W (formerly known as Watermelon mosaic virus 1, WMV-1). Different combinations of growth regulators were evaluated for the regeneration of cotyledon explants. Adventitious buds or shoot primordia were obtained within 3 to 4 weeks on regeneration medium. After shoot development, adventitious buds or shoot primordia were transferred to elongation medium for 3 to 4 weeks and these shoots were subcultured onto rooting medium for another 1 to 2 weeks. Under optimal culture conditions, a total of 7 to 10 weeks was necessary to obtain C. metuliferus plantlets from cotyledons. Furthermore, transgenic plants were successfully obtained using an Agrobacterium tumefaciens-mediated transformation method as shown by polymerase chain reaction analysis and histochemical beta-glucuronidase (GUS) assay. A total of nine transgenic plants were developed from 360 cotyledon explants, giving a transformation frequency of 2.5%.
ISSN: 0018-5345
Appears in Collections:植物病理學系

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