Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/46152
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dc.contributor.authorChen, K.C.en_US
dc.contributor.author葉錫東zh_TW
dc.contributor.authorChiang, C.H.en_US
dc.contributor.authorRaja, J.A.J.en_US
dc.contributor.authorLiu, F.L.en_US
dc.contributor.authorTai, C.H.en_US
dc.contributor.authorYeh, S.D.en_US
dc.date2008zh_TW
dc.date.accessioned2014-06-06T08:18:50Z-
dc.date.available2014-06-06T08:18:50Z-
dc.identifier.issn0894-0282zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/46152-
dc.description.abstractMost strains of Papaya ringspot virus (PRSV) belong to type W, causing severe loss on cucurbits worldwide, or type P, devastating papaya in tropical areas. While the host range of PRSV W is limited to plants of the families Chenopodiaceae and Cucuribitaceae, PRSV P, in addition, infects plants of the family Caricaceae (papaya family). To investigate one or more viral genetic determinants for papaya infection, recombinant viruses were constructed between PRSV P-YK and PRSV W-Cl. Host reactions to recombinant viruses indicated that the viral genomic region covering the C-terminal region (142 residues) of NIaVPg, full NIaPro, and N-terminal region (18 residues) of NIb, is critical for papaya infection. Sequence analysis of this region revealed residue variations at position 176 of NIaVPg and positions 27 and 205 of NIaPro between type P and W viruses. Host reactions to the constructed mutants indicated that the amino acid Lys(27) of NIaPro determines the host-specificity of PRSV for papaya infection. Predicted three-dimensional structures of NIaPros of parental viruses suggested that Lys(27) does not affect the protease activity of NIaPro. Recovery of the infected plants from certain papaya-infecting mutants implied involvement of other viral factors for enhancing virulence and adaptation of PRSV on papaya.en_US
dc.language.isoen_USzh_TW
dc.relationMolecular Plant-Microbe Interactionsen_US
dc.relation.ispartofseriesMolecular Plant-Microbe Interactions, Volume 21, Issue 8, Page(s) 1046-1057.en_US
dc.relation.urihttp://dx.doi.org/10.1094/mpmi-21-8-1046en_US
dc.subjecthost-specificity determinanten_US
dc.subjectwatermelon mosaic virus-1en_US
dc.subjectcylindrical-inclusion proteinen_US
dc.subjectfactor iso 4een_US
dc.subjectviral proteinen_US
dc.subjectactive-siteen_US
dc.subjectpotyvirusen_US
dc.subjecttranslationen_US
dc.subjectvpgen_US
dc.subjectrnaen_US
dc.subjectresistanceen_US
dc.titleA single amino acid of NIaPro of Papaya ringspot virus determines host specificity for infection of papayaen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1094/mpmi-21-8-1046zh_TW
item.grantfulltextnone-
item.openairetypeJournal Article-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:植物病理學系
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