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|標題:||Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers virus resistance||作者:||Niu, Q.W.
|關鍵字:||yellow-mosaic-virus;silencing-mediated defense;rna interference;gene;plants;protein;targets;recombination;replication;suppressor||Project:||Nature Biotechnology||期刊/報告no：:||Nature Biotechnology, Volume 24, Issue 11, Page(s) 1420-1428.||摘要:||
Plant microRNAs ( miRNAs) regulate the abundance of target mRNAs by guiding their cleavage at the sequence complementary region. We have modified an Arabidopsis thaliana miR159 precursor to express artificial miRNAs ( amiRNAs) targeting viral mRNA sequences encoding two gene silencing suppressors, P69 of turnip yellow mosaic virus ( TYMV) and HC- Pro of turnip mosaic virus ( TuMV). Production of these amiRNAs requires A. thaliana DICER- like protein 1. Transgenic A. thaliana plants expressing amiR- P69(159) and amiR- HC- Pro(159) are specifically resistant to TYMV and TuMV, respectively. Expression of amiR-TuCP(159) targeting TuMV coat protein sequences also confers specific TuMV resistance. However, transgenic plants that express both amiR- P69(159) and amiR- HC- Pro(159) from a dimeric pre- amiR- P69(159)/ amiR- HC- Pro(159) transgene are resistant to both viruses. The virus resistance trait is displayed at the cell level and is hereditable. More important, the resistance trait is maintained at 15 degrees C, a temperature that compromises small interfering RNA - mediated gene silencing. The amiRNA- mediated approach should have broad applicability for engineering multiple virus resistance in crop plants.
|Appears in Collections:||植物病理學系|
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