Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/46154
DC FieldValueLanguage
dc.contributor.authorNiu, Q.W.en_US
dc.contributor.author葉錫東zh_TW
dc.contributor.authorLin, S.S.en_US
dc.contributor.authorReyes, J.L.en_US
dc.contributor.authorChen, K.C.en_US
dc.contributor.authorWu, H.W.en_US
dc.contributor.authorYeh, S.D.en_US
dc.contributor.authorChua, N.H.en_US
dc.date2006zh_TW
dc.date.accessioned2014-06-06T08:18:50Z-
dc.date.available2014-06-06T08:18:50Z-
dc.identifier.issn1087-0156zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/46154-
dc.description.abstractPlant microRNAs ( miRNAs) regulate the abundance of target mRNAs by guiding their cleavage at the sequence complementary region. We have modified an Arabidopsis thaliana miR159 precursor to express artificial miRNAs ( amiRNAs) targeting viral mRNA sequences encoding two gene silencing suppressors, P69 of turnip yellow mosaic virus ( TYMV) and HC- Pro of turnip mosaic virus ( TuMV). Production of these amiRNAs requires A. thaliana DICER- like protein 1. Transgenic A. thaliana plants expressing amiR- P69(159) and amiR- HC- Pro(159) are specifically resistant to TYMV and TuMV, respectively. Expression of amiR-TuCP(159) targeting TuMV coat protein sequences also confers specific TuMV resistance. However, transgenic plants that express both amiR- P69(159) and amiR- HC- Pro(159) from a dimeric pre- amiR- P69(159)/ amiR- HC- Pro(159) transgene are resistant to both viruses. The virus resistance trait is displayed at the cell level and is hereditable. More important, the resistance trait is maintained at 15 degrees C, a temperature that compromises small interfering RNA - mediated gene silencing. The amiRNA- mediated approach should have broad applicability for engineering multiple virus resistance in crop plants.en_US
dc.language.isoen_USzh_TW
dc.relationNature Biotechnologyen_US
dc.relation.ispartofseriesNature Biotechnology, Volume 24, Issue 11, Page(s) 1420-1428.en_US
dc.relation.urihttp://dx.doi.org/10.1038/nbt1255en_US
dc.subjectyellow-mosaic-virusen_US
dc.subjectsilencing-mediated defenseen_US
dc.subjectrna interferenceen_US
dc.subjectgeneen_US
dc.subjectplantsen_US
dc.subjectproteinen_US
dc.subjecttargetsen_US
dc.subjectrecombinationen_US
dc.subjectreplicationen_US
dc.subjectsuppressoren_US
dc.titleExpression of artificial microRNAs in transgenic Arabidopsis thaliana confers virus resistanceen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1038/nbt1255zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
Appears in Collections:植物病理學系
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