Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/46164
標題: Isolation and characterization of the third gene encoding a 16.9 kDa class I low-molecular-mass heat shock protein, Oshsp 16.9C, in rice
作者: Chang, P.F.L.
張碧芳
Huang, C.Y.
Chang, F.C.
Tseng, T.S.
Lin, W.C.
Lin, C.Y.
關鍵字: heat shock protein;heat shock gene;low-molecular-mass heat shock;protein;Oryza sativa L.;oryza-sativa;soybean seedlings;plants;thermotolerance;activation;expression;sequence;promoter;invitro;vitro
Project: Botanical Bulletin of Academia Sinica
期刊/報告no:: Botanical Bulletin of Academia Sinica, Volume 42, Issue 2, Page(s) 85-92.
摘要: 
Using rice cDNA pTS1 encoding a 16.9 kDa heat shock protein (HSP) as a probe for Southern hybridization analysis, we observed five prominent bands of 9.5, 5.9, 3.4, 2.5, and 1.7 kb in the Eco RI digests of rice genomic DNA and found them to contain six individual genes. The 5.9 kb DNA fragment was further digested with Hind m to generate three fragments of 3.5, 1.7 and 0.7 kb, and we found, using pTS1 cDNA as a probe, the 3.5 kb fragment contained a putative low-molecular-mass (LMM) HSP gene. The DNA sequencing of 3.5 kb fragment revealed encoding of a presumptive 16.9 kDa HSP (149 amino acid residues) with predicted pi value of 6.42. The nucleotide sequence of this gene was highly homologous to the coding regions of two rice class I LMM HSP genes, Oshsp, 16.9A and Oshsp16.9B, sharing 93.1% and 94.3% sequence identity, respectively, as published previously in our laboratory. The deduced amino acid sequence of this gene is similar to those of the Oshsp16.9A and Oshsp16.9B genes with a difference of only ii and 10 amino acids, respectively. It was hence designated as Oshsp16.9C (accession no. U81385). We used 3' UTRs (untranslated regions) for analysis of Oshsp16.9C gene expression since the 3' UTRs of these three genes showed Very low sequence homology.
URI: http://hdl.handle.net/11455/46164
ISSN: 0006-8063
Appears in Collections:植物病理學系

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