Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/46164
DC FieldValueLanguage
dc.contributor.authorChang, P.F.L.en_US
dc.contributor.author張碧芳zh_TW
dc.contributor.authorHuang, C.Y.en_US
dc.contributor.authorChang, F.C.en_US
dc.contributor.authorTseng, T.S.en_US
dc.contributor.authorLin, W.C.en_US
dc.contributor.authorLin, C.Y.en_US
dc.date2001zh_TW
dc.date.accessioned2014-06-06T08:18:51Z-
dc.date.available2014-06-06T08:18:51Z-
dc.identifier.issn0006-8063zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/46164-
dc.description.abstractUsing rice cDNA pTS1 encoding a 16.9 kDa heat shock protein (HSP) as a probe for Southern hybridization analysis, we observed five prominent bands of 9.5, 5.9, 3.4, 2.5, and 1.7 kb in the Eco RI digests of rice genomic DNA and found them to contain six individual genes. The 5.9 kb DNA fragment was further digested with Hind m to generate three fragments of 3.5, 1.7 and 0.7 kb, and we found, using pTS1 cDNA as a probe, the 3.5 kb fragment contained a putative low-molecular-mass (LMM) HSP gene. The DNA sequencing of 3.5 kb fragment revealed encoding of a presumptive 16.9 kDa HSP (149 amino acid residues) with predicted pi value of 6.42. The nucleotide sequence of this gene was highly homologous to the coding regions of two rice class I LMM HSP genes, Oshsp, 16.9A and Oshsp16.9B, sharing 93.1% and 94.3% sequence identity, respectively, as published previously in our laboratory. The deduced amino acid sequence of this gene is similar to those of the Oshsp16.9A and Oshsp16.9B genes with a difference of only ii and 10 amino acids, respectively. It was hence designated as Oshsp16.9C (accession no. U81385). We used 3' UTRs (untranslated regions) for analysis of Oshsp16.9C gene expression since the 3' UTRs of these three genes showed Very low sequence homology.en_US
dc.language.isoen_USzh_TW
dc.relationBotanical Bulletin of Academia Sinicaen_US
dc.relation.ispartofseriesBotanical Bulletin of Academia Sinica, Volume 42, Issue 2, Page(s) 85-92.en_US
dc.subjectheat shock proteinen_US
dc.subjectheat shock geneen_US
dc.subjectlow-molecular-mass heat shocken_US
dc.subjectproteinen_US
dc.subjectOryza sativa L.en_US
dc.subjectoryza-sativaen_US
dc.subjectsoybean seedlingsen_US
dc.subjectplantsen_US
dc.subjectthermotoleranceen_US
dc.subjectactivationen_US
dc.subjectexpressionen_US
dc.subjectsequenceen_US
dc.subjectpromoteren_US
dc.subjectinvitroen_US
dc.subjectvitroen_US
dc.titleIsolation and characterization of the third gene encoding a 16.9 kDa class I low-molecular-mass heat shock protein, Oshsp 16.9C, in riceen_US
dc.typeJournal Articlezh_TW
item.fulltextno fulltext-
item.grantfulltextnone-
item.openairetypeJournal Article-
item.languageiso639-1en_US-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
Appears in Collections:植物病理學系
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