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標題: RNA polyrnerase II gene (RPB2) encoding the second largest protein subunit in Phaeosphaeria nodorum and P-avenaria
作者: Malkus, A.
Chang, P.F.L.
Zuzga, S.M.
Chung, K.R.
Shao, J.
Cunfer, B.M.
Arseniuk, E.
Ueng, P.P.
關鍵字: Cochliobolus heterostrophus;enzymes;plant pathology;Stagnospora;nodorum;Stagnospora leaf blotch;small-grain cereals;mating-type genes;polymerase-ii;molecular;phylogeny;septoria diseases;sequence diversity;beta-tubulin;evolution;stagonospora;fungus
Project: Mycological Research
期刊/報告no:: Mycological Research, Volume 110, Page(s) 1152-1164.
A 5586 bp sequence (accession no. DQ278491), which includes the RNA polymerase II gene (RPB2) encoding the second largest protein subunit (RPB2), was obtained from the wheat biotype Phaeosphaeria nodorum (PN-w) by PCR amplification. The 3841 bp full length RPB2 gene contains two exons and a 52 bp intron, and encodes a complete 1262 amino acid protein. Similar to the C-terminals of the beta subunits of prokaryotes and yeast RNA polymerases, the deduced RPB2 protein contained many structural features needed for gene transcription. Based on the phylogenetic analysis with the deduced RPB2 polypeptide sequences, the PN-w was closely related to the maize pathogen Cochliobolus heterostrophus. Size differences were found in the full length RPB2 gene of cereal Phaeosphaeria species, mainly due to differences in intron size. No nucleotide substitutions were found in homothallic P. avenaria f.sp. triticea (Pat1) and barley biotype P. nodorum (PN-b) isolates used in this study. The nucleotide and deduced amino acid sequences of the RPB2 gene in Pat1 were closely related to that in PN-w. (c) 2006 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
ISSN: 0953-7562
DOI: 10.1016/j.mycres.2006.07.015
Appears in Collections:植物病理學系

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