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|標題:||A chlorotic spot disease on calla lilies (Zantedeschia spp.) is caused by a tospovirus serologically but distantly related to Watermelon silver mottle virus||作者:||Chen, C.C.
|關鍵字:||electron microscopy;insect vector;serology;wilt-like virus;genome sequence;l rna;identification;completion;proteins||Project:||Plant Disease||期刊/報告no：:||Plant Disease, Volume 89, Issue 5, Page(s) 440-445.||摘要:||
A new tospovirus, Calla lily chlorotic spot virus (CCSV), was isolated front calla lilies (Zanledeschia spp.) in Taiwan. Chlorotic spots, ranging from light green to yellow, appear on the middle leaves of the affected plants. Virions measuring 75 to 105 rim. similar in size to tospovirus particles, were present in crude extracts and ultrathin sections of diseased leaves. Of 35 plant species inoculated mechanically, 24, including wax gourd (Benincasa hispida) and zucchini squash (Cucurbita pepo), were susceptible to the virus. CCSV was transmitted from infected wax gourd by Thrips palmi to healthy wax gourd and zucchini squash. The virus was weakly related to Watermelon silver mottle virus (WSMoV) in enzyme-linked immunosorbent assay (ELISA) and western blot tests. WSMoV-specific N gene printers, however, failed to produce DNA fragments from total RNA extracts of CCSV-infected plants in reverse transcription-polymerase chain reaction (RT-PCR). Results of RT-PCR show that the conserved regions of the L genes of tospoviruses are present in CCSV.
|Appears in Collections:||植物病理學系|
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