Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/46196
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dc.contributor.authorChen, C.C.en_US
dc.contributor.author葉錫東zh_TW
dc.contributor.authorChen, T.C.en_US
dc.contributor.authorRaja, J.A.en_US
dc.contributor.authorChang, C.A.en_US
dc.contributor.authorChen, L.W.en_US
dc.contributor.authorLin, S.S.en_US
dc.contributor.authorYeh, S.D.en_US
dc.date2007zh_TW
dc.date.accessioned2014-06-06T08:18:54Z-
dc.date.available2014-06-06T08:18:54Z-
dc.identifier.issn0168-1702zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/46196-
dc.description.abstractThe N-terminal (NT) regions of particular protein-coding sequences are generally used for in-frame insertion of heterologous open reading frames (ORFs) in potyviral vectors for protein expression in plants. An infectious cDNA clone of Turnip mosaic virus (TuMV) isolate YC5 was engineered at the generally used NT regions of HC-Pro and CP, and other possibly permissive sites to investigate their effectiveness to express the GFP (jellyfish green fluorescent protein) and Der p 5 (allergen from the dust mite, Dermatophagoides pteronyssinus) ORFs. The results demonstrated the permissiveness of the NT regions of P3, CIP and NIb to carry the ORFs and express the translates as part of the viral polyprotein, the processing of which released free-form proteins in the host cell milieu. However, these sites varied in their permissiveness to retain the ORFs intact and hence affect the heterologous protein expression. Moreover, strong influence of the inserted ORF and host plants in determining the permissiveness of a viral genomic context to stably carry the alien ORFs and hence to support their prolonged expression was also noticed. In general, the engineered sites were relatively more permissive to the GFP ORF than to the Der p 5 ORF. Among the hosts, the local lesion host, Chenopodium quinoa Willd. showed the highest extent of support to TuMV to stably carry the heterologous ORFs at the engineered sites and the protein expression therefrom. Among the systemic hosts, Nicotiana benthamiana Domin proved more supportive to TuMV to carry and express the heterologous ORFs than the Brassica hosts, whereas the protein expression levels were significantly higher and more stable in the plants of Brassica campestris L. var. chinensis and B. campestris L. var. ching-geeng than those in the plants of B. juncea L. and B. campestris L. var. pekinensis. (c) 2007 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationVirus Researchen_US
dc.relation.ispartofseriesVirus Research, Volume 130, Issue 1-2, Page(s) 210-227.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.virusres.2007.06.014en_US
dc.subjectTuMV vectoren_US
dc.subjectpotyvirusen_US
dc.subjectplant expressionen_US
dc.subjectdust mite allergen Der p 5en_US
dc.subjectinsertion sitesen_US
dc.subjectstabilityen_US
dc.subjecthelper component-proteinaseen_US
dc.subjecttobacco etch potyvirusen_US
dc.subjectlong-distanceen_US
dc.subjectmovementen_US
dc.subjectto-cell movementen_US
dc.subjectcapsid proteinen_US
dc.subjectbeta-glucuronidaseen_US
dc.subjectmolecular-biologyen_US
dc.subjectescherichia-colien_US
dc.subjectforeign proteinsen_US
dc.subjectraphanus-sativusen_US
dc.titleEffectiveness and stability of heterologous proteins expressed in plants by Turnip mosaic virus vector at five different insertion sitesen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.virusres.2007.06.014zh_TW
item.languageiso639-1en_US-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.openairetypeJournal Article-
item.fulltextno fulltext-
item.grantfulltextnone-
Appears in Collections:植物病理學系
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