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標題: Genetic analysis of an attenuated Papaya ringspot virus strain applied for cross-protection
作者: Chiang, C.H.
Lee, C.Y.
Wang, C.H.
Jan, F.J.
Lin, S.S.
Chen, T.C.
Raja, J.A.J.
Yeh, S.D.
關鍵字: attenuated virus;cross-protection;in vitro transcription;recombinant;virus;turnip-mosaic-virus;plum-pox-virus;helper component-proteinase;vein;mottling virus;mutational analysis;plant-virus;pathogenicity;determinants;multifunctional protein;nucleotide-sequence;symptom;expression
Project: European Journal of Plant Pathology
期刊/報告no:: European Journal of Plant Pathology, Volume 118, Issue 4, Page(s) 333-348.
Papaya ringspot virus (PRSV) HA 5-1, a nitrous acid-induced mild mutant of severe strain HA, widely applied for control of PRSV by cross-protection, was used to study the genetic basis of attenuation. Using infectious clones, a series of recombinants was generated between HA 5-1 and HA and their infectivity was analyzed on the systemic host papaya and the local lesion host Chenopodium quinoa. The recombinants that contained mutations in PI and HC-Pro genes caused attenuated infection on papaya without conspicuous symptoms, similar to HA 5-1. The recombination and sequence analyses strongly implicated two amino acid changes in the C-terminal region of PI and two in HC-Pro of HA 5-1 involved in the attenuated infection on papaya. The recombinants that infected C. quinoa plants without local lesions contained the same mutations in the C-terminal region of HC-Pro for attenuated infection on papaya. We conclude that both PI and HC-Pro bear important pathogenicity determinants for the infection on the systemic host papaya and that the mutations in HC-Pro affecting pathogenicity on papaya are also responsible for the inability to induce hypersensitive reaction on C. quinoa.
ISSN: 0929-1873
DOI: 10.1007/s10658-007-9130-z
Appears in Collections:植物病理學系

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