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|標題:||Genetic analysis of an attenuated Papaya ringspot virus strain applied for cross-protection||作者:||Chiang, C.H.
|關鍵字:||attenuated virus;cross-protection;in vitro transcription;recombinant;virus;turnip-mosaic-virus;plum-pox-virus;helper component-proteinase;vein;mottling virus;mutational analysis;plant-virus;pathogenicity;determinants;multifunctional protein;nucleotide-sequence;symptom;expression||Project:||European Journal of Plant Pathology||期刊/報告no：:||European Journal of Plant Pathology, Volume 118, Issue 4, Page(s) 333-348.||摘要:||
Papaya ringspot virus (PRSV) HA 5-1, a nitrous acid-induced mild mutant of severe strain HA, widely applied for control of PRSV by cross-protection, was used to study the genetic basis of attenuation. Using infectious clones, a series of recombinants was generated between HA 5-1 and HA and their infectivity was analyzed on the systemic host papaya and the local lesion host Chenopodium quinoa. The recombinants that contained mutations in PI and HC-Pro genes caused attenuated infection on papaya without conspicuous symptoms, similar to HA 5-1. The recombination and sequence analyses strongly implicated two amino acid changes in the C-terminal region of PI and two in HC-Pro of HA 5-1 involved in the attenuated infection on papaya. The recombinants that infected C. quinoa plants without local lesions contained the same mutations in the C-terminal region of HC-Pro for attenuated infection on papaya. We conclude that both PI and HC-Pro bear important pathogenicity determinants for the infection on the systemic host papaya and that the mutations in HC-Pro affecting pathogenicity on papaya are also responsible for the inability to induce hypersensitive reaction on C. quinoa.
|Appears in Collections:||植物病理學系|
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