Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/46214
DC FieldValueLanguage
dc.contributor.authorChiang, C.H.en_US
dc.contributor.author詹富智zh_TW
dc.contributor.authorLee, C.Y.en_US
dc.contributor.authorWang, C.H.en_US
dc.contributor.authorJan, F.J.en_US
dc.contributor.authorLin, S.S.en_US
dc.contributor.authorChen, T.C.en_US
dc.contributor.authorRaja, J.A.J.en_US
dc.contributor.authorYeh, S.D.en_US
dc.contributor.author葉錫東zh_TW
dc.date2007zh_TW
dc.date.accessioned2014-06-06T08:18:55Z-
dc.date.available2014-06-06T08:18:55Z-
dc.identifier.issn0929-1873zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/46214-
dc.description.abstractPapaya ringspot virus (PRSV) HA 5-1, a nitrous acid-induced mild mutant of severe strain HA, widely applied for control of PRSV by cross-protection, was used to study the genetic basis of attenuation. Using infectious clones, a series of recombinants was generated between HA 5-1 and HA and their infectivity was analyzed on the systemic host papaya and the local lesion host Chenopodium quinoa. The recombinants that contained mutations in PI and HC-Pro genes caused attenuated infection on papaya without conspicuous symptoms, similar to HA 5-1. The recombination and sequence analyses strongly implicated two amino acid changes in the C-terminal region of PI and two in HC-Pro of HA 5-1 involved in the attenuated infection on papaya. The recombinants that infected C. quinoa plants without local lesions contained the same mutations in the C-terminal region of HC-Pro for attenuated infection on papaya. We conclude that both PI and HC-Pro bear important pathogenicity determinants for the infection on the systemic host papaya and that the mutations in HC-Pro affecting pathogenicity on papaya are also responsible for the inability to induce hypersensitive reaction on C. quinoa.en_US
dc.language.isoen_USzh_TW
dc.relationEuropean Journal of Plant Pathologyen_US
dc.relation.ispartofseriesEuropean Journal of Plant Pathology, Volume 118, Issue 4, Page(s) 333-348.en_US
dc.relation.urihttp://dx.doi.org/10.1007/s10658-007-9130-zen_US
dc.subjectattenuated virusen_US
dc.subjectcross-protectionen_US
dc.subjectin vitro transcriptionen_US
dc.subjectrecombinanten_US
dc.subjectvirusen_US
dc.subjectturnip-mosaic-virusen_US
dc.subjectplum-pox-virusen_US
dc.subjecthelper component-proteinaseen_US
dc.subjectveinen_US
dc.subjectmottling virusen_US
dc.subjectmutational analysisen_US
dc.subjectplant-virusen_US
dc.subjectpathogenicityen_US
dc.subjectdeterminantsen_US
dc.subjectmultifunctional proteinen_US
dc.subjectnucleotide-sequenceen_US
dc.subjectsymptomen_US
dc.subjectexpressionen_US
dc.titleGenetic analysis of an attenuated Papaya ringspot virus strain applied for cross-protectionen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1007/s10658-007-9130-zzh_TW
item.openairetypeJournal Article-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en_US-
Appears in Collections:植物病理學系
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