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|標題:||The gene coding for the Hrp pilus structural protein is required for type III secretion of Hrp and Avr proteins in Pseudomonas syringae pv. tomato||作者:||Wei, W.S.
|關鍵字:||alternate sigma-factor;yersinia ysc homologs;target-cell contact;hypersensitive response;epithelial-cells;salmonella-typhimurium;pathogenic bacteria;negative regulation;escherichia-coli;expression||Project:||Proceedings of the National Academy of Sciences of the United States of America||期刊/報告no：:||Proceedings of the National Academy of Sciences of the United States of America, Volume 97, Issue 5, Page(s) 2247-2252.||摘要:||
Bacterial surface appendages called pili often are associated with DNA and/or protein transfer between cells. The exact function of pill in the transfer process is not understood and is a matter of considerable debate. The Hrp pilus is assembled by the Hrp type III protein secretion system of Pseudomonas syringae pv. tomato (Pst) strain DC3000. In this study, we show that the hrpA gene, which encodes the major subunit of the Hrp pilus, is required for secretion of putative virulence proteins, such as HrpW and AvrPto. In addition, the hrpA gene is required for full expression of genes that encode regulatory, secretion, and effector proteins of the type III secretion system. hrpA-mediated gene regulation apparently is through effect on the mRNA level of two previously characterized regulatory genes, hrpR and hrpS, Ectopic expression of the hrpRS gene operon restored gene expression, but not protein secretion, in the hrpA mutant. Three single amino acid mutations at the HrpA carboxyl terminus were identified that affect the secretion or regulatory function of the HrpA protein. These results define an essential role of the Hrp pilus structural gene in protein secretion and coordinate regulation of the type III secretion system in Pst DC3000.
|Appears in Collections:||植物病理學系|
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