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標題: Development of a molecular method for rapid differentiation of watermelon lines resistant to Fusarium oxysporum f. sp niveum
作者: Lin, Y.H.
Chen, K.S.
Liou, T.D.
Huang, J.W.
Chang, P.F.L.
關鍵字: Breeding programs;Citrullus lanatus;Fusarium oxysporum f. sp niveum;Fusarium wilt;Molecular detection;Molecular marker;PCR;RAPD;SCAR;wilt resistance;dna marker;identification;gene;primers;region;locus;fom-2;aflp
Project: Botanical Studies
期刊/報告no:: Botanical Studies, Volume 50, Issue 3, Page(s) 273-280.
The vascular wilt disease of watermelon caused by Fusarium oxysporum f. sp. niveum (Fon) is one of the major factors limiting watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai) production worldwide. Breeding highly resistant lines is one of the best ways to control the disease. In order to accelerate the breeding programs, a reliable PCR (polymerase chain reaction) technique was developed to rapidly differentiate the disease-resistant watermelons. The G05-SCAR (sequence characterized amplified region) primer set GsF5/GsR5, derived from the OP-G05 random primer-amplified fragment sequence, produced an 898 bp DNA fragment which was specific to watermelon lines resistant or tolerant to Fon. With optimized PCR assay, the Molecular method could easily differentiate Fon-resistant and -susceptible hybrid watermelon populations.
ISSN: 1817-406X
Appears in Collections:植物病理學系

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