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標題: Development of a molecular marker for specific detection of Fusarium oxysporum f. sp cubense race 4
作者: Lin, Y.H.
Chang, J.Y.
Liu, E.T.
Chao, C.P.
Huang, J.W.
Chang, P.F.L.
關鍵字: Molecular detection;Panama disease;PCR;RAPD;Reliable diagnosis;vegetative compatibility groups;pcr assays;cavendish banana;panama-disease;wilt;culmorum;graminearum;resistance;diversity;avenaceum
Project: European Journal of Plant Pathology
期刊/報告no:: European Journal of Plant Pathology, Volume 123, Issue 3, Page(s) 353-365.
Fusarium oxysporum f. sp. cubense is the causal agent of Panama disease of banana. A rapid and reliable diagnosis is the foundation of integrated disease management practices in commodity crops. For this diagnostic purpose, we have developed a reliable molecular method to detect Foc race 4 isolates in Taiwan. By PCR amplification, the primer set Foc1/Foc-2 derived from the sequence of a random primer OP-A02 amplified fragment produced a 242 bp size DNA fragment which was specific to Foc race 4. With the optimized PCR parameters, the molecular method was sensitive and could detect small quantities of Foc DNA as low as 10 pg in 50 to 2,000 ng host genomic DNA with high efficiency. We also demonstrated that by using our PCR assay with Foc-1/Foc-2 primer set, Foc race 4 could be easily distinguished from other Foc races 1 and 2, and separated other formae speciales of F. oxysporum.
ISSN: 0929-1873
DOI: 10.1007/s10658-008-9372-4
Appears in Collections:植物病理學系

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