Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/4915
DC FieldValueLanguage
dc.contributor.advisor盧至人zh_TW
dc.contributor.author郭育仁zh_TW
dc.date2002zh_TW
dc.date.accessioned2014-06-06T06:33:32Z-
dc.date.available2014-06-06T06:33:32Z-
dc.identifier.urihttp://hdl.handle.net/11455/4915-
dc.description.abstract本實驗研究的目的,乃利用熱篩法或甲烷菌抑制劑(BES)的添加,將甲烷菌篩除,探討混合族群中非甲烷菌在四氯乙烯厭氧脫氯的過程中所扮演的角色。結果發現,當BES濃度由0 mM增加至200 mM,四氯乙烯的代謝不但沒有被抑制,反而具有促進的效果,顯示混合族群中的非甲烷族群,具有較佳的脫氯效果。當硫酸鹽濃度由5mM增加至20 mM時,熱篩組與未熱篩組都顯示出硫酸鹽對於脫氯的現象並無助益,而當硫酸鹽濃度由50 mM增加至200 mM時,熱篩組與未熱篩組都顯示出硫酸鹽對於脫氯的具有抑制的現象。硫酸鹽還原菌計數試驗中也並未觀察到硫酸鹽還原菌的菌落,顯示混合族群中並無硫酸鹽還原菌的存在。添加200 mM BES後,加入不同濃度的乳酸量(0∼20 mM)的實驗中,可以發現隨著乳酸量的提高,四氯乙烯的初始代謝率及總代謝量大幅增加,顯示在無甲烷菌及硫酸鹽還原菌的狀態下,產氫菌及利用氫氣行鹵化呼吸作用(halorespiration)的脫氯菌可能為混合族群中,代謝四氯乙烯的主要菌種。藉由100 mM BES的添加後,加入乳酸及相等於乳酸量的醋酸鈉及氫氣做比較,以釐清產氫菌及鹵化呼吸菌,何者為混合族群中代謝四氯乙烯的主要菌種,結果顯示本族群在無甲烷菌及硫酸鹽還原菌的狀態下,四氯乙烯以產氫菌的代謝約佔5%,以鹵化呼吸菌的脫氯約佔95%。zh_TW
dc.description.abstractIn this study, the heat pretreatment and the addition of inhibitor (BES) were used to sieve methanogenic cells and then to confirm the anaerobic dechlorination of tetrachloroethylene (PCE) by non-methanogenic cells. With the addition of BES from 0 to 200 (mM), it was found that BES did not inhibit the dechlorination of tetrachloroethylene. On the contrary, the addition of BES was helpful to enhance the dechlorination of PCE. The experimental results showed the dechlorination of PCE by non-methanogenics was superior to methanogenics. For both of the heat pretreatment and non-heat pretreatment of cells, the dechlorination of PCE did not increase with the increase of SO42- from 5 to 20 (mM). However, when the concentrations of sulfate increased from 50 to 200 (mM), the dechlorination of PCE was inhibited. In the experiment of sulfate-reducing bacteria count, sulfate-reducing bacteria were not found in this mixed culture. After the addition of 200 (mM) BES, the PCE removal rate increased significantly as the concentrations of lactate increased from 0 to 20 (mM). The experimental results indicated that hydrogen-producing bacteria and halorespiring bacteria played the major role in the dechlorination of PCE, but not methanogenic or sulfate-reducing bacteria. In order to understand the cells responsible for the dechlorination of PCE, lactate or both acetate and hydrogen were used as the substrate. The experimental results indicated that the dechlorination of PCE resulted from both hydrogen-producing bacteria and halorespiring bacteria. However, halorespiring bacteria played the most significant role for the dechlorination of PCE. More than 95% of PCE dechlorination resulted from halorespiring bacteria.en_US
dc.description.tableofcontents摘 要 1 Abstract 2 目錄 4 圖目錄 6 表目錄 11 第一章 前言 12 1-1研究緣起 13 1-2 研究目的 14 第二章 文獻回顧 15 2-1地下水的重要性 15 2-2含氯碳氫化合物對地下水之污染 17 2-3四氯乙烯之物化特性 19 2-4四氯乙烯的毒性及其代謝途徑 21 2-5地下水污染整治技術 27 2-5-1地下水抽取處理系統 ( pump-and-treat system ) 27 2-5-2地下水生物復育 28 2-5-3植物復育法 ( Phytoremediation ) 29 2-5-4天然衰減法 29 2-6厭氧生物處理之基本機制 30 2-7基質產氫效應 32 2-8硫酸鹽還原菌作用機制 33 2-9甲烷菌作用機制 35 2-10四氯乙烯在環境中之轉換 36 2-10-1四氯乙烯厭氧生物代謝機制 38 2-10-2四氯乙烯厭氧生物代謝形式 39 2-10-3各菌種脫氯能力的比較 46 2-11菌種在厭氧脫氯上之研究 48 第三章 實驗設備與方法 55 3-1實驗用藥品 55 3-1-1實驗用水 55 3-1-2主要基質 55 3-1-3氫氣(H2) 56 3-1-4含氯碳氫化合物 56 3-1-5 無機營養鹽 57 3-1-6硫酸 58 3-1-7 HPLC沖提液 58 3-1-8 IC沖提液 59 3-1-9 丙酮 59 3-1-10 甲烷抑制劑 60 3-1-11 硫酸鹽還原菌計數試驗之營養物 60 3-2 菌種 60 3-2-1 菌種來源 60 3-2-2 菌種培養及馴化 60 3-3 分析設備與分析方法 61 3-3-1 四氯乙烯與三氯乙烯之分析 61 3-3-2 二氯乙烯與氯乙烯之分析 65 3-3-3 甲烷及乙烯之分析 66 3-3-4氫氣之分析 66 3-3-5 醋酸鈉與乳酸之分析 68 3-3-6 硫酸根之分析 68 3-3-7 pH之測定 69 3-4 實驗方法與步驟 69 3-4-1 檢量線的建立 69 3-4-2 MLVSS測定方法 71 3-4-3 批次實驗流程及準備事項 72 3-4-4硫酸鹽還原菌計數試驗(李,1992) 77 第四章 結果與討論 79 4-1 背景實驗 79 4-1-1 四氯乙烯之非生物性轉換 79 4-1-2 氫氣之非生物性轉換 79 4-2 抑制劑濃度效應 80 4-2-1抑制劑濃度對甲烷混合族群之影響 83 4-3 死菌效應 95 4-3-1 不添加任何基質之批次試驗 96 4-3-2 添加不同濃度死菌液之試驗 96 4-4熱篩法對四氯乙烯厭氧分解之效應 106 4-4-1 最佳熱篩溫度選擇試驗 107 4-2-2 等量熱篩試驗 107 4-4 硫酸鹽還原菌試驗 116 4-4-1 硫酸鹽添加試驗 117 4-4-2 硫酸鹽還原菌計數 141 4-5 產氫菌及鹵化呼吸菌試驗 144 4-5-1 乳酸添加試驗 145 4-5-2 等量添加試驗 152 第五章 結論及建議 157 5-1結論 157 5-2 建議 159 第六章 參考文獻 161zh_TW
dc.language.isozh_TWzh_TW
dc.publisher環境工程學系zh_TW
dc.subject四氯乙烯zh_TW
dc.subject熱篩法zh_TW
dc.subject硫酸鹽還原菌zh_TW
dc.subject產氫菌zh_TW
dc.subject鹵化呼吸菌zh_TW
dc.title厭氧生物處理四氯乙烯代謝方式之探討zh_TW
dc.typeThesis and Dissertationzh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeThesis and Dissertation-
item.cerifentitytypePublications-
item.fulltextno fulltext-
item.languageiso639-1zh_TW-
item.grantfulltextnone-
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