Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50295
DC FieldValueLanguage
dc.contributor.authorWu, C.Y.en_US
dc.contributor.author徐堯煇zh_TW
dc.contributor.authorYang, S.H.en_US
dc.contributor.authorLai, Y.C.en_US
dc.contributor.authorLin, N.S.en_US
dc.contributor.authorHsu, Y.H.en_US
dc.contributor.authorHu, C.C.en_US
dc.date2007zh_TW
dc.date.accessioned2014-06-06T08:49:15Z-
dc.date.available2014-06-06T08:49:15Z-
dc.identifier.issn0168-1702zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/50295-
dc.description.abstractReplication of genomic DNAs of plant-pathogenic begomoviruses has been demonstrated in prokaryotes, which supported the possibility of analyzing DNA replication process of begomoviruses in bacteria. However, previous studies indicated that the replication of begomovirus DNAs in prokaryotes requires tandem constructs of viral genomes with at least two copies of the origin of replication (ori). In this study, phage M13 vector harboring the unit-length genome with only a single copy of ori of a mono-partite begomovirus, Ageratum yellow vein virus PD isolate (AYVV-[PD]), was constructed and used to investigate the replication of AYVV-[PD] DNAs in Escherichia coli. The generation of single-stranded, circular DNAs (sscDNAs) corresponding to the unit-length AYVV-[PD] genome of both polarity was observed and verified. Replication-associated (Rep) protein of AYVV-[PD] was detected only in bacteria generating the corresponding sscDNAs, whereas disruption of the Rep gene abolished the phenomenon. The results suggested that a single copy of ori is sufficient for the prokaryotes to support the generation of unit-length, genomic sscDNAs of begomoviruses, which requires the presence of functional Rep protein. (c) 2006 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationVirus Researchen_US
dc.relation.ispartofseriesVirus Research, Volume 125, Issue 1, Page(s) 14-28.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.virusres.2006.12.001en_US
dc.subjectbegomovirusesen_US
dc.subjectEscherichia colien_US
dc.subjectphage M13en_US
dc.subjectreplication-associateden_US
dc.subjectproteinen_US
dc.subjectsingle-stranded circular DNAen_US
dc.subjectcassava-mosaic-virusen_US
dc.subjectrolling-circleen_US
dc.subjectsaccharomyces-cerevisiaeen_US
dc.subjectplant-cellsen_US
dc.subjectreverse-transcriptaseen_US
dc.subjectdependent replicationen_US
dc.subjectgeminivirusen_US
dc.subjectproteinen_US
dc.subjectrnaen_US
dc.subjectsequencesen_US
dc.titleUnit-length, single-stranded circular DNAs of both polarity of begomoviruses are generated in Escherichia coli harboring phage M13-cloned begomovirus genome with single copy of replication originen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.virusres.2006.12.001zh_TW
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:生物科技學研究所
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