Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50302
DC FieldValueLanguage
dc.contributor.authorTsai, C.H.en_US
dc.contributor.author蔡慶修zh_TW
dc.contributor.authorCheng, C.P.en_US
dc.contributor.authorPeng, C.W.en_US
dc.contributor.authorLin, B.Y.en_US
dc.contributor.authorLin, N.S.en_US
dc.contributor.authorHsu, Y.H.en_US
dc.contributor.author鄭綺萍zh_TW
dc.contributor.author徐堯煇zh_TW
dc.date1999zh_TW
dc.date.accessioned2014-06-06T08:49:16Z-
dc.date.available2014-06-06T08:49:16Z-
dc.identifier.issn0022-538Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/50302-
dc.description.abstractRNAs transcribed from a full-length infectious cDNA clone of the bamboo mosaic potexvirus (strain O) genome, pBaMV-O, were infectious to Nicotiana benthamiana plants. Mutant genomes in which the poly(A) tail is absent or replaced by a 3' tRNA-like structure from turnip yellow mosaic virus RNA failed to amplify detectably in N. benthamiana protoplasts. No amplification was detected in protoplasts inoculated with transcripts containing 4, 7, or 10 adenylate residues at the 3' end, whereas transcript inocula with 15 adenylate residues resulted in coat protein accumulation to a level 26% of that resulting from inoculation with transcripts with 25 adenylate residues (designated as wild type). Coat protein accumulation levels of 69 and 98% relative to wild type were observed after inoculation of]protoplasts with transcripts bearing poly(A) tails 18 and 22 nucleotides long, respectively. The presence of a putative 3' pseudoknot structure including at least 13 adenylate residues of the 3'-terminal poly(A) tail was supported by enzymatic and chemical structural analysis. The functional relevance of this putative pseudoknot was tested by mutations that affected basepairing within the pseudoknot. These results support the existence of functional 3' pseudoknot that includes part of the 3' poly(A) tail.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Virologyen_US
dc.relation.ispartofseriesJournal of Virology, Volume 73, Issue 4, Page(s) 2703-2709.en_US
dc.subjectpotato-virus-xen_US
dc.subjectnucleotide-sequenceen_US
dc.subjectcloned cdnaen_US
dc.subjectviral-rnaen_US
dc.subjectinfectiousen_US
dc.subjecttranscriptsen_US
dc.subjectregionen_US
dc.subjectgenomeen_US
dc.subjectsiteen_US
dc.subjectsubstitutionsen_US
dc.subjectmutagenesisen_US
dc.titleSufficient length of a poly(A) tail for the formation of a potential pseudoknot is required for efficient replication of bamboo mosaic potexvirus RNAen_US
dc.typeJournal Articlezh_TW
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:生物科技學研究所
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