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|標題:||A desiccation-induced transcript in lily (Lilium longiflorum) pollen||作者:||Ko, C.W.
|關鍵字:||ABA-inducible;cDNA;desiccation;Lilium longiflorum;pollen-specific;protein-patterns;gene-expression;messenger-rnas;stress;acid;tolerance;sequence;anther||Project:||Journal of Plant Physiology||期刊/報告no：:||Journal of Plant Physiology, Volume 159, Issue 7, Page(s) 765-772.||摘要:||
This work characterizes a desiccation and abscisic acid (ABA)-induced transcript, designated LLP-B3, present in pollen of Lilium longiflorum during development and stress. The LLP-B3 cDNA encodes a gene product having a sequence of 160 amino acids, a calculated molecular mass of 17.8 kDa, and a pl of 4.3. The polypeptide is mainly hydrophilic having a stretch of hydrophobic region at the N-terminus, indicating the presence of a signal peptide. The expression of LLP-B3 gene is pollen-specific, and the transcript accumulates only at the stage of pollen maturation. Premature drying of developing pollen indicated that the accumulation of LLP-B3 transcripts was associated with desiccation. Treatment of pollen with abscisic acid resulted in the accumulation of LLP-B3 mRNA during germination. Sequence analysis demonstrates similarities between the predicted lily LLP-B3 protein and a corn Zmc13, a tomato LAT52, an arabidopsis leaf SAH7 and a group of major allergens including Ole e 1 of olive tree. Southern blots of lily genomic; DNA indicate that LLP-B3 is a single or low copy number gene. The function of the desiccation-associated protein is discussed.
|Appears in Collections:||生物科技學研究所|
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