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http://hdl.handle.net/11455/50308
標題: | A desiccation-induced transcript in lily (Lilium longiflorum) pollen | 作者: | Ko, C.W. 王國祥 Yang, C.Y. Wang, C.S. |
關鍵字: | ABA-inducible;cDNA;desiccation;Lilium longiflorum;pollen-specific;protein-patterns;gene-expression;messenger-rnas;stress;acid;tolerance;sequence;anther | Project: | Journal of Plant Physiology | 期刊/報告no:: | Journal of Plant Physiology, Volume 159, Issue 7, Page(s) 765-772. | 摘要: | This work characterizes a desiccation and abscisic acid (ABA)-induced transcript, designated LLP-B3, present in pollen of Lilium longiflorum during development and stress. The LLP-B3 cDNA encodes a gene product having a sequence of 160 amino acids, a calculated molecular mass of 17.8 kDa, and a pl of 4.3. The polypeptide is mainly hydrophilic having a stretch of hydrophobic region at the N-terminus, indicating the presence of a signal peptide. The expression of LLP-B3 gene is pollen-specific, and the transcript accumulates only at the stage of pollen maturation. Premature drying of developing pollen indicated that the accumulation of LLP-B3 transcripts was associated with desiccation. Treatment of pollen with abscisic acid resulted in the accumulation of LLP-B3 mRNA during germination. Sequence analysis demonstrates similarities between the predicted lily LLP-B3 protein and a corn Zmc13, a tomato LAT52, an arabidopsis leaf SAH7 and a group of major allergens including Ole e 1 of olive tree. Southern blots of lily genomic; DNA indicate that LLP-B3 is a single or low copy number gene. The function of the desiccation-associated protein is discussed. |
URI: | http://hdl.handle.net/11455/50308 | ISSN: | 0176-1617 | DOI: | 10.1078/0176-1617-0749 |
Appears in Collections: | 生物科技學研究所 |
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