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標題: Identification of anther-specific/predominant genes regulated by gibberellin during development of lily anthers
作者: Hsu, Y.F.
Tzeng, A.D.
Liu, M.C.
Yei, F.L.
Chung, M.C.
Wang, C.S.
關鍵字: anther;early genes;gibberellin;lily (Lilium longiflorum);suppression;subtractive hybridization;arabidopsis-thaliana;male gametophyte;expression;pollen;biosynthesis;lycopersicon;tapetum;rice
Project: Journal of Plant Physiology
期刊/報告no:: Journal of Plant Physiology, Volume 165, Issue 5, Page(s) 553-563.
We successfully identify anther-specific/predominant genes induced by gibberellin (GA) at the microspore stage of lily (Lilium longiflorum) anthers. We used a suppression-subtractive hybridization strategy to identify 22 individual cDNAs followed by a reverse RNA dot plot to determine their specificities at the microspore stage. Of the 22 genes, 12 are clearly anther-specific and three are anther-predominant. Sequence analysis revealed that five anther-specific/predominant genes are novel. The transcripts of anther-specific/predominant genes were differentially detected at the microspore development phase; some began accumulating in level as early as the occurrence of meiosis. When uniconazole, an inhibitor of GA biosynthesis was applied in young lily plants we found that all of the anther-specific/predominant genes, with the exception of LLA-139, were up-regulated by GAs in the anther while only some were responsive to the exogenous addition of 100 mu M GA(3). In situ hybridization with antisense riboprobes of selected genes in the anther showed a strong signal localized to the tapetal. layer. The different actions of GA on gene expression in anthers are discussed. (c) 2007 Elsevier GrnbH. All rights reserved.
ISSN: 0176-1617
DOI: 10.1016/j.jplph.2007.01.008
Appears in Collections:生物科技學研究所

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