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標題: A lily pollen ASR protein localizes to both cytoplasm and nuclei requiring a nuclear localization signal
作者: Wang, H.J.
Hsu, C.M.
Jauh, G.Y.
Wang, C.S.
關鍵字: lilium-longiflorum;gene;arabidopsis;expression;plants;grape;cells;sugar;rna
Project: Physiologia Plantarum
期刊/報告no:: Physiologia Plantarum, Volume 123, Issue 3, Page(s) 314-320.
LLA23, an abscisic acid-, stress- and ripening-induced (ASR) protein, was isolated previously from lily (Lilium longiflorum) pollen. Close examination of the C-terminus of this ASR protein revealed the presence of basic regions reminiscent of a nuclear localization signal (NLS). Fluorescence microscopy studies using green fluorescent protein (GFP) fusion proteins indicated that the bipartite NLS in LLA23 exhibited nuclear localization properties. Accordingly, mutations in the NLS motifs of LLA23 defined two regions, either of which was necessary for partial nuclear targeting and both of which were required for complete nuclear localization. In addition, oligonucleotide-directed mutagenesis identified lysine residues within the NLS necessary for nuclear localization. Immunogold localization confirmed that the protein was located to both the cytoplasm and nucleus of generative and vegetative cells of pollen grains; the generative nuclei showed the highest number of LLA23 labelling. The possible function of ASR proteins in both the cytoplasm and nuclei of pollen grains is discussed.
ISSN: 0031-9317
DOI: 10.1111/j.1399-3054.2005.00454.x
Appears in Collections:生物科技學研究所

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