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|標題:||A unique caleosin in oil bodies of lily pollen||作者:||Jiang, P.L.
|關鍵字:||caleosin;lily (Lilium longiflorum thunb.);oil bodies;oleosin;pollen;binding protein;lipid bodies;germination;biogenesis;oleosin;plants;seeds||Project:||Plant and Cell Physiology||期刊/報告no：:||Plant and Cell Physiology, Volume 49, Issue 9, Page(s) 1390-1395.||摘要:||
In view of the recent isolation of stable oil bodies as well as a unique oleosin from lily pollen, this study examined whether other minor proteins were present in this lipid-storage organelle. Immunological cross-recognition using antibodies against three minor oil-body proteins from sesame suggested that a putative caleosin was specifically detected in the oil-body fraction of pollen extract. A cDNA fragment encoding this putative pollen caleosin, obtained by PCR cloning, was confirmed by immunodetection and MALDI-MS analyses of the recombinant protein over-expressed in Escherichia coli and the native form. Caleosin in lily pollen oil bodies seemed to be a unique isoform distinct from that in lily seed oil bodies.
|Appears in Collections:||生物科技學研究所|
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