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標題: A unique caleosin in oil bodies of lily pollen
作者: Jiang, P.L.
Jauh, G.Y.
Wang, C.S.
Tzen, J.T.C.
關鍵字: caleosin;lily (Lilium longiflorum thunb.);oil bodies;oleosin;pollen;binding protein;lipid bodies;germination;biogenesis;oleosin;plants;seeds
Project: Plant and Cell Physiology
期刊/報告no:: Plant and Cell Physiology, Volume 49, Issue 9, Page(s) 1390-1395.
In view of the recent isolation of stable oil bodies as well as a unique oleosin from lily pollen, this study examined whether other minor proteins were present in this lipid-storage organelle. Immunological cross-recognition using antibodies against three minor oil-body proteins from sesame suggested that a putative caleosin was specifically detected in the oil-body fraction of pollen extract. A cDNA fragment encoding this putative pollen caleosin, obtained by PCR cloning, was confirmed by immunodetection and MALDI-MS analyses of the recombinant protein over-expressed in Escherichia coli and the native form. Caleosin in lily pollen oil bodies seemed to be a unique isoform distinct from that in lily seed oil bodies.
ISSN: 0032-0781
DOI: 10.1093/pcp/pcn103
Appears in Collections:生物科技學研究所

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