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標題: PCR method for detecting recombinant baculoviruses with the insertion of a large gene
作者: Lai, S.Y.
Ho, J.Y.
Wang, M.Y.
關鍵字: expression vectors;virus
Project: Biotechnology Techniques
期刊/報告no:: Biotechnology Techniques, Volume 12, Issue 10, Page(s) 733-736.
A PCR strategy was developed using primers specific to an infectious bursal disease virus (IBDV) gene as well as primers flanking the polyhedrin region of baculovirus to verify the presence of IBDV gene in the recombinant baculovirus and confirm the absence of wild-type baculovirus contamination. This method can be applied to detect the presence of large genes in the recombinant baculovirus with greater sensitivity and avoid the need of modifying the typical PCR procedure provided by the manufacturer.
ISSN: 0951-208X
DOI: 10.1023/a:1008875525844
Appears in Collections:生物科技學研究所

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