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|標題:||Identification of the RNA-binding sites of the triple gene block protein 1 of bamboo mosaic potexvirus||作者:||Wung, C.H.
|關鍵字:||virus movement protein;polymerase chain-reaction;stranded;nucleic-acids;to-cell transport;subcellular-localization;nucleotide-sequence;overlap extension;genomic rna;in-vitro;domain||Project:||Journal of General Virology||期刊/報告no：:||Journal of General Virology, Volume 80, Page(s) 1119-1126.||摘要:||
The triple gene block protein 1 (TGBp1) encoded by open reading frame 2 of bamboo mosaic potexvirus (BaMV) was overexpressed in Escherichia coli and purified in order to test its RNA-binding activity. UV crosslinking assays revealed that the RNA-binding activity was present mainly in the soluble fraction of the refolded TGBp1. The binding activity was nonspecific and salt concentration-dependent: activity was present at 0-50 mM NaCl but was almost abolished at 200 mM. The RNA-binding domain was located by deletion mutagenesis to the N-terminal 3-24 amino acids of TGBp1. Sequence alignment analysis of the N-terminal 25 amino acids of the TGBp1 homologues of potexviruses identified three arginine residues. Arg-to-Ala substitution at any one of the three arginines eliminated most of the RNA-binding activity, indicating that they were all critical to the RNA-binding activity of the TGBp1 of BaMV.
|Appears in Collections:||生物科技學研究所|
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