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標題: A simplified method of constructing infectious clones of begomovirus employing limited restriction enzyme digestion of products of rolling circle amplification
作者: Wu, C.Y.
Lai, Y.C.
Lin, N.S.
Hsu, Y.H.
Tsai, H.T.
Liao, J.Y.
Hu, C.C.
關鍵字: begomovirus;infectious clone;limited restriction enzyme digestion;rolling circle amplification;yellow vein disease;golden mosaic-virus;leaf-curl-virus;streak virus;ageratum-conyzoides;dna-polymerase;viral-dna;replication;plants;genome
Project: Journal of Virological Methods
期刊/報告no:: Journal of Virological Methods, Volume 147, Issue 2, Page(s) 355-359.
Most infectious clones of geminiviruses consist of (partial) tandem repeats of viral genomes in the vectors, which usually involve tedious, multi-step assemblies of genomic fragments in the construction process. A simplified procedure was devised to circumvent these problems, which employs limited restriction digestion of multimeric viral genomes produced by rolling circle amplification (RCA), followed by direct cloning into appropriate vectors. The efficiency of the procedure, and infectivity of the dimeric constructs it produced, were demonstrated using three different geminiviruses, namely ageratum yellow vein virus, tomato leaf curl virus, and squash leaf curl virus. (c) 2007 Elsevier B.V. All rights reserved.
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2007.10.002
Appears in Collections:生物科技學研究所

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