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標題: One-step reverse transcription loop-mediated isothermal amplification assay for rapid detection of Cymbidium mosaic virus
作者: Lee, M.S.
Yang, M.J.
Hseu, Y.C.
Lai, G.H.
Chang, W.T.
Hsu, Y.H.
Lin, M.K.
Project: Journal of Virological Methods
期刊/報告no:: Journal of Virological Methods, Volume 173, Issue 1, Page(s) 43-48.
Cymbidium mosaic virus (CymMV) is the most prevalent orchid virus. A single-tube one-step betaine-free reverse transcription (RI) loop-mediated isothermal amplification (LAMP) assay was developed for the rapid and easy detection of orchid-infecting CymMV. Five sets of primers were designed based on the conserved regions among various virus isolates. The specificity and the sensitivity of the assay were then evaluated using the RI-LAMP reaction. Within 1 h under isothermal conditions at 60 degrees C the target viral gene was amplified successfully. This RT-LAMP assay was found to be quick, specific, sensitive and easy to perform assay that involved only one step and was simpler to carry out than alternative approaches. Thus this assay is an alternative for the rapid and easy detection of CymMV in orchids. This is first time that a RI-LAMP method for the detection of an orchid virus has been described. (C) 2011 Elsevier B.V. All rights reserved.
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2011.01.005
Appears in Collections:生物科技學研究所

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