Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50375
DC FieldValueLanguage
dc.contributor.authorPalani, P.V.en_US
dc.contributor.author徐堯煇zh_TW
dc.contributor.authorKasiviswanathan, V.en_US
dc.contributor.authorChen, J.C.F.en_US
dc.contributor.authorChen, W.en_US
dc.contributor.authorHsu, Y.H.en_US
dc.contributor.authorLin, N.S.en_US
dc.date2006zh_TW
dc.date.accessioned2014-06-06T08:49:23Z-
dc.date.available2014-06-06T08:49:23Z-
dc.identifier.issn0894-0282zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/50375-
dc.description.abstractSatellite RNA of Bamboo mosaic virus (satBaMV) has a single open reading frame for a nonstructural, RNA-binding protein, P20, which facilitates the long-distance movement of satBaMV in Nicotiana benthamiana. Here, we elucidate various biological properties of P20 and the involvement of a single domain in its activities. P20 displayed a strong self-interaction in vitro and in vivo, and cross-linking assays demonstrated its oligomerization. Domain mapping, using the bacterial two-hybrid system, indicated that the self-interacting domain overlaps the RNA-binding domain in the N-terminal arginine-rich motif (ARM) of P20. The deletion of the ARM abolished the self-interaction of P20 in vitro and in vivo and impaired its intracellular targeting and efficient cell-to-cell movement in N. benthamiana leaves. Moreover, RNA and protein accumulation of the ARM deletion mutant of satBaMV was significantly reduced in leaves systemically coinfected with Bamboo mosaic potexvirus and satBaMV. This is the first report of the involvement of ARM in various biological activities of a satellite RNA-encoded protein during infection of its host.en_US
dc.language.isoen_USzh_TW
dc.relationMolecular Plant-Microbe Interactionsen_US
dc.relation.ispartofseriesMolecular Plant-Microbe Interactions, Volume 19, Issue 7, Page(s) 758-767.en_US
dc.relation.urihttp://dx.doi.org/10.1094/mpmi-19-0758en_US
dc.subjectprotein-protein interactionen_US
dc.subjecttriple-gene-blocken_US
dc.subjectyellow-vein-virusen_US
dc.subjectbinding domainen_US
dc.subjectsubcellular-localizationen_US
dc.subjectnucleotide-sequenceen_US
dc.subjectcapsid proteinen_US
dc.subjectviral-rnaen_US
dc.subjectpotexvirusen_US
dc.subjectreplicationen_US
dc.subjectplasmodesmataen_US
dc.titleThe arginine-rich motif of Bamboo mosaic virus satellite RNA-encoded P20 mediates self-interaction, intracellular targeting, and cell-to-cell movementen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1094/mpmi-19-0758zh_TW
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
Appears in Collections:生物科技學研究所
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