Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50376
DC FieldValueLanguage
dc.contributor.authorHsu, Y.H.en_US
dc.contributor.author徐堯煇zh_TW
dc.contributor.authorAnnamalai, P.en_US
dc.contributor.authorLin, C.S.en_US
dc.contributor.authorChen, Y.Y.en_US
dc.contributor.authorChang, W.C.en_US
dc.contributor.authorLin, N.S.en_US
dc.date2000zh_TW
dc.date.accessioned2014-06-06T08:49:23Z-
dc.date.available2014-06-06T08:49:23Z-
dc.identifier.issn0032-0862zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/50376-
dc.description.abstractA sensitive method was used to detect bamboo mosaic virus (BaMV) and its associated satellite RNA (satBaMV) by P-32- and digoxigenin (Dig)-labelled probes synthesized from cDNA clones of BaMV genomic (L probe) and satBaMV (S probe) RNA. Both the P-32- and Dig-labelled L and S probes could detect as little as 490 pg of BaMV viral RNA by slot- and dot-blot hybridization. In infected leaf extracts, P-32-labelled L and S probes detected virus at 25-fold higher dilutions than Dig-labelled probes, which were also successfully used to detect BaMV infection in plants derived from meristem-tip culture. However, immunoassays failed to detect BaMV in meristem culture. By dot-blot hybridization assays, 25% of the seedlings were shown to be virus-free. These results suggest that a highly sensitive method for the detection of BaMV infection is required for the establishment of BaMV-free cultures. Meristem-tip culture also provides an efficient method for obtaining virus-free bamboo plants.en_US
dc.language.isoen_USzh_TW
dc.relationPlant Pathologyen_US
dc.relation.ispartofseriesPlant Pathology, Volume 49, Issue 1, Page(s) 101-107.en_US
dc.subjectbamboo mosaic potexvirusen_US
dc.subjectDig-labelled probeen_US
dc.subjectmeristem cultureen_US
dc.subjectradioactive and nonradioactive hybridizationen_US
dc.subjectriboprobeen_US
dc.subjectsatellite RNAen_US
dc.subjectlabeled rna probesen_US
dc.subjectsatellite rnaen_US
dc.subjectplant-virusesen_US
dc.subjectnucleotide-sequenceen_US
dc.subjectpotexvirusen_US
dc.subjecteliminationen_US
dc.subjecthybridizationen_US
dc.subjectregenerationen_US
dc.subjectreplicationen_US
dc.subjectorchidsen_US
dc.titleA sensitive method for detecting bamboo mosaic virus (BaMV) and establishment of BaMV-free meristem-tip culturesen_US
dc.typeJournal Articlezh_TW
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:生物科技學研究所
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