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標題: A sensitive method for detecting bamboo mosaic virus (BaMV) and establishment of BaMV-free meristem-tip cultures
作者: Hsu, Y.H.
Annamalai, P.
Lin, C.S.
Chen, Y.Y.
Chang, W.C.
Lin, N.S.
關鍵字: bamboo mosaic potexvirus;Dig-labelled probe;meristem culture;radioactive and nonradioactive hybridization;riboprobe;satellite RNA;labeled rna probes;satellite rna;plant-viruses;nucleotide-sequence;potexvirus;elimination;hybridization;regeneration;replication;orchids
Project: Plant Pathology
期刊/報告no:: Plant Pathology, Volume 49, Issue 1, Page(s) 101-107.
A sensitive method was used to detect bamboo mosaic virus (BaMV) and its associated satellite RNA (satBaMV) by P-32- and digoxigenin (Dig)-labelled probes synthesized from cDNA clones of BaMV genomic (L probe) and satBaMV (S probe) RNA. Both the P-32- and Dig-labelled L and S probes could detect as little as 490 pg of BaMV viral RNA by slot- and dot-blot hybridization. In infected leaf extracts, P-32-labelled L and S probes detected virus at 25-fold higher dilutions than Dig-labelled probes, which were also successfully used to detect BaMV infection in plants derived from meristem-tip culture. However, immunoassays failed to detect BaMV in meristem culture. By dot-blot hybridization assays, 25% of the seedlings were shown to be virus-free. These results suggest that a highly sensitive method for the detection of BaMV infection is required for the establishment of BaMV-free cultures. Meristem-tip culture also provides an efficient method for obtaining virus-free bamboo plants.
ISSN: 0032-0862
Appears in Collections:生物科技學研究所

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