Please use this identifier to cite or link to this item:
標題: Characterization of Oil Bodies in Adlay (Coix lachryma-jobi L)
作者: Lu, H.C.
Jiang, P.L.
Hsu, L.R.C.
Chyan, C.L.
Tzen, J.T.C.
關鍵字: adlay;caleosin;Coix lachryma-jobi;oleosin;seed oil bodies;oleosin isoforms;lily pollen;caleosin;proteins;seeds;germination;expression;cloning;rice;bran
Project: Bioscience Biotechnology and Biochemistry
期刊/報告no:: Bioscience Biotechnology and Biochemistry, Volume 74, Issue 9, Page(s) 1841-1847.
Oil bodies were observed in cells of both embryo and aleurone layers of mature adlay grains (Coix lachryma-jobi L. var. ma-yuen Stapf). Stable oil bodies were successfully isolated from the adlay grains. Thin-layer chromatography revealed that the contents stored in the adlay oil bodies were mainly neutral lipids (>90% triacylglycerols and about 5% diacylglycerols). The integrity of the isolated oil bodies was presumably maintained via electronegative repulsion and steric hindrance provided by their surface proteins. Immunological cross-recognition using antibodies against sesame oil-body proteins indicated that two oleosin isoforms (termed oleosin-H and oleosin-L) and one caleosin were present in the adlay oil bodies. Full-length cDNA fragments encoding these three unique oil-body proteins were obtained by PCR cloning. MALDI-MS analyses confirmed that the three full-length cDNA fragments encoded the two oleosin isoforms and one caleosin observed in the oil bodies isolated from the adlay grains.
ISSN: 0916-8451
DOI: 10.1271/bbb.100247
Appears in Collections:生物科技學研究所

Show full item record

Google ScholarTM




Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.