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標題: Gene family of oleosin isoforms and their structural stabilization in sesame seed oil bodies
作者: 曾志正
Tai, S.S.K.
Chen, M.C.M.
Peng, C.C.
Tzen, J.T.C.
關鍵字: oil body;oleosin isoform;seed;sesame;structural stability;safflower carthamus-tinctorius;arabidopsis-thaliana;proteins;surface;cloning;maize;classification;expression;cotyledons
Project: Bioscience Biotechnology and Biochemistry
期刊/報告no:: Bioscience Biotechnology and Biochemistry, Volume 66, Issue 10, Page(s) 2146-2153.
Oleosins are structural proteins sheltering the oil bodies of plant seeds. Two isoform classes termed H- and L-oleosin are present in diverse angiosperms. Two H-oleosins and one L-oleosin were identified in sesame oil bodies from the protein sequences deduced from their corresponding cDNA clones. Sequence analysis showed that the main difference between the H- and L-isoforms is an insertion of 18 residues in the C-terminal domain of H-oleosins. H-oleosin, presumably derived from L-oleosin, was duplicated independently in several species. All known oleosins can be classified as one of these two isoforms. Single copy or a low copy number was detected by Southern hybridization for each of the three oleosin genes in the sesame genome. Northern hybridization showed that the three oleosin genes were transcribed in maturing seeds where oil bodies are being assembled. Artificial oil bodies were reconstituted with triacylglycerol, phospholipid, and sesame oleosin isoforms. The results indicated that reconstituted oil bodies could be stabilized by both isoforms, but L-oleosin gave slightly more structural stability than H-oleosin.
ISSN: 0916-8451
Appears in Collections:生物科技學研究所

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