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|標題:||Molecular cloning of the precursor polypeptide of mastoparan B and its putative processing enzyme, dipeptidyl peptidase IV, from the black-bellied hornet, Vespa basalis||作者:||曾志正
|關鍵字:||dipeptidyl peptidase IV;mastoparan B;propeptide;venom toxin;Vespa;basalis;honeybee prepromelittin;crystal-structure;melittin;venom;aminopeptidase;membranes;mechanism;sequence;protein||Project:||Insect Molecular Biology||期刊/報告no：:||Insect Molecular Biology, Volume 16, Issue 2, Page(s) 231-237.||摘要:||
Mastoparan B, a cationic toxin, is the major peptide component in the venom of Vespa basalis. Molecular cloning of its cDNA fragment revealed that this toxin was initially synthesized as a precursor polypeptide, containing an N-terminal signal sequence, a prosequence, the mature toxin, and an appendix glycine at C-terminus. Sequence alignment between precursors of mastoparan B and melittin from honeybee venom showed a significant conservation in prosequence. Alternate positions existing in both prosequences were either proline or alanine known as the potential cleaving sites for dipeptidyl peptidase IV. Subsequently, a putative dipeptidyl peptidase IV cDNA fragment was cloned from Vespa basalis venom gland. The prosequence may possibly be removed via sequential liberation of dipeptides during the processing of mastoparan B.
|Appears in Collections:||生物科技學研究所|
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