Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50428
DC FieldValueLanguage
dc.contributor.authorChen, J.C.F.en_US
dc.contributor.author曾志正zh_TW
dc.contributor.authorTsai, C.C.Y.en_US
dc.contributor.authorTzen, J.T.C.en_US
dc.date1999zh_TW
dc.date.accessioned2014-06-06T08:49:29Z-
dc.date.available2014-06-06T08:49:29Z-
dc.identifier.issn0032-0781zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/50428-
dc.description.abstractPlant seed oil bodies comprise a matrix of triacylglycerols surrounded by a monolayer of phospholipids embedded with abundant oleosins and some minor proteins. Three minor proteins, temporarily termed Sops 1-3, have been identified in sesame oil bodies. A cDNA sequence of Sop1 was obtained by PCR cloning using degenerate primers derived from two partial amino acid sequences, and subsequently confirmed via immunological recognition of its over-expressed protein in Escherichia coli, Alignment with four published homologous sequences suggests Sop1 as a putative calcium-binding protein, Immunological cross-recognition implies that this protein, tentatively named caleosin, exists in diverse seed oil bodies, Caleosin migrated faster in SDS-PAGE when incubated with Ca2+. A single copy of caleosin gene was found in sesame genome based on Southern hybridization. Northern hybridization revealed that both caleosin and oleosin genes were concurrently transcribed in maturing seeds where oil bodies are actively assembled, Hydropathy plot and secondary structure analysis suggest that caleosin comprises three structural domains, i.e,, an N-terminal hydrophilic calcium-binding domain, a central hydrophobic anchoring domain, and a C-terminal hydrophilic phosphorylation domain. Compared with oleosin, a conserved proline knot-like motif is located in the central hydrophobic domain of caleosin and assumed to involve in protein assembly onto oil bodies.en_US
dc.language.isoen_USzh_TW
dc.relationPlant and Cell Physiologyen_US
dc.relation.ispartofseriesPlant and Cell Physiology, Volume 40, Issue 10, Page(s) 1079-1086.en_US
dc.subjectcaleosinen_US
dc.subjectoil bodyen_US
dc.subjectproline knoten_US
dc.subjectseeden_US
dc.subjectsesameen_US
dc.subjectoleosinen_US
dc.subjectsurfaceen_US
dc.subjectbiogenesisen_US
dc.subjecttopologyen_US
dc.subjectsesameen_US
dc.titleCloning and secondary structure analysis of caleosin, a unique calcium-binding protein in oil bodies of plant seedsen_US
dc.typeJournal Articlezh_TW
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
Appears in Collections:生物科技學研究所
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