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標題: Stable oil bodies sheltered by a unique caleosin in cycad megagametophytes
作者: Jiang, P.L.
Chen, J.C.F.
Chiu, S.T.
Tzen, J.T.C.
關鍵字: Caleosin;Cycad (Cycas revoluta);Megagametophyte;Oil bodies;Oleosin;lily pollen;oleosin;genes;seeds;proteins;accumulation;germination;expression;size
Project: Plant Physiology and Biochemistry
期刊/報告no:: Plant Physiology and Biochemistry, Volume 47, Issue 11-12, Page(s) 1009-1016.
Stable oil bodies of smaller sizes and higher thermostability were isolated from mature cycad (Cycas revoluta) megagametophytes compared with those isolated from sesame seeds. Immunological cross-recognition revealed that cycad oil bodies contained a major protein of 27 kDa, tentatively identified as caleosin, while oleosin, the well-known structural protein, was apparently absent. Mass spectrometric analysis showed that the putative cycad caleosin possessed a tryptic fragment of 15 residues matching to that of a theoretical moss caleosin. A complete cDNA fragment encoding this putative caleosin was obtained by PCR cloning using a primer designed according to the tryptic peptide and another one designed according to a highly conservative region among diverse caleosins. The identification of this clone was subsequently confirmed by immunodetection and MALDI-MS analyses of its recombinant fusion protein over-expressed in Escherichia coli and the native form from cycad oil bodies. Stable artificial oil bodies were successfully constituted with triacylglycerol, phospholipid and the recombinant fusion protein containing the cycad caleosin. These results suggest that stable oil bodies in cycad megagametophytes are mainly sheltered by a unique structural protein caleosin. (C) 2009 Elsevier Masson SAS. All rights reserved.
ISSN: 0981-9428
DOI: 10.1016/j.plaphy.2009.07.004
Appears in Collections:生物科技學研究所

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