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|標題:||An orchid (Oncidium Gower Ramsey) AP3-like MADS gene regulates floral formation and initiation||作者:||Hsu, H.F.
|關鍵字:||Arabidopsis thaliana;floral formation;floral initiation;MADS box;genes;Oncidium Gower Ramsey;homeotic gene;flower development;box genes;arabidopsis-thaliana;ectopic expression;organ identity;molecular characterization;shoot;development;multigene family;dna-binding||Project:||Plant and Cell Physiology||期刊/報告no：:||Plant and Cell Physiology, Volume 43, Issue 10, Page(s) 1198-1209.||摘要:||
cDNA for a B group MADS box gene OMADS3 was isolated and characterized from Oncidium Gower Ramsey, an important species of orchid. OMADS3 encoding a 204 amino acid protein showed high sequence homology to both paleoAP3 and TM6 lineage of B group MADS box gene such as monocots AP3 homologue LMADS1 in lily and GDEF1 in Gerbera hybrida. Despite the sequence homology, consensus motifs identified in the C-terminal region of B group genes were absent in OMADS3. Southern analysis indicated that OMADS3 was present in O. Gower Ramsey genome in low copy numbers. Different from most B group genes, OMADS3 mRNA was detected in all four floral organs as well as in vegetative leaves. This is similar to the expression pattern of GDEF1. 35S::OMADS3 transgenic plants showed novel phenotypes by producing terminal flowers similar to those observed in transgenic plants ectopically expressed A functional genes such as AP1. Ectopic expression of OMADS3 cDNA truncated with the MADS box or C terminal region in Arabidopsis generated novel ap2-like flowers in which sepals and petals were converted into carpel-like and stamen-like structures. Yeast two-hybrid analysis indicated that OMADS3 is able to strongly form homodimers. Our results suggested that OMADS3 might represent an ancestral form of TM6-like gene which was conserved in monocots with a function similar to A functional gene in regulating flower formation as well as floral initiation.
|Appears in Collections:||生物科技學研究所|
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