Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50445
標題: EMF genes interact with late-flowering genes in regulating floral initiation genes during shoot development in Arabidopsis thaliana
作者: Chou, M.L.
楊長賢
Haung, M.D.
Yang, C.H.
關鍵字: APETALA1;Arabidopsis thaliana;embryonic flower mutant;floral;transition;late-flowering mutants;LEAFY;time genes;inflorescence development;reproductive development;meristem identity;fld interacts;leafy;apetala1;activation;encodes;protein
Project: Plant and Cell Physiology
期刊/報告no:: Plant and Cell Physiology, Volume 42, Issue 5, Page(s) 499-507.
摘要: 
To investigate the mechanisms regulating the initiation of floral development in Arabidopsis, a construct containing beta -glucuronidase (GUS) gene driven by APETALA1 promoter (AP1::GUS) was introduced into emf fwa and emf ft double mutants. GUS activity was strongly detected on shoot meristem of emf1-1 Single mutants harboring AP1:GUS construct just 5 d after germination. By contrast, GUS activity was undetectable on emf1-1 fwa-1, emf1-1 ft-1, emf2-1 fwa-1, emf2-3 fwa-1 and emf2-3 ft-1 double mutants harboring API::GUS construct 10 d after germination, GUS activity was only weakly detected on the apical meristem of 20-day-old emf1-1 fwa-1 and emf2-1 fn,a-l seedlings. During this time, only sessile leaves were produced. Further analysis indicated that API was strongly expressed in 10-day-old emf1-1 and emf2-1 Single mutants. Its expression was significantly reduced in all emf1-1 or emf2-1 late-flowering double mutants tested. Similar to API, the expression of LEA FY(LFY) was also high in emf1-1 and emf2-1 single mutants and reduced in emf1-1 or emf2-1 late-flowering double mutants. Our results indicate that the precocious expression of API and LFY is dependent not only on the low EMF and FWA activities but also on the expression of most of the late-flowering genes such as FT, FCA, FE, CO and GI,These data also reveal that most late-flowering genes may function downstream of EMF or in pathways distinct from EI)IF to activate genes specified floral meristem identity during shoot maturation in Arabidopsis.
URI: http://hdl.handle.net/11455/50445
ISSN: 0032-0781
DOI: 10.1093/pcp/pce062
Appears in Collections:生物科技學研究所

Show full item record
 

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.