Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50465
DC FieldValueLanguage
dc.contributor.authorChiu, M.H.en_US
dc.contributor.author蔡慶修zh_TW
dc.contributor.authorChen, I.H.en_US
dc.contributor.authorBaulcombe, D.C.en_US
dc.contributor.authorTsai, C.H.en_US
dc.date2010zh_TW
dc.date.accessioned2014-06-06T08:49:33Z-
dc.date.available2014-06-06T08:49:33Z-
dc.identifier.issn1464-6722zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/50465-
dc.description.abstractP>Previous evidence has indicated that the P25 protein encoded by Potato virus X (PVX) inhibits either the assembly or function of the effector complexes in the RNA silencing-based antiviral defence system (Bayne et al., Cell-to-cell movement of Potato Potexvirus X is dependent on suppression of RNA silencing. Plant J. 44, 471-482). This finding prompted us to investigate the possibility that P25 targets the Argonaute (AGO) effector nuclease of RNA silencing. Co-immunoprecipitation and Western blot analysis indicated that there is a strong interaction between P25 and AGO1 of Arabidopsis when these proteins are transiently co-expressed in Nicotiana benthamiana. P25 also interacts with AGO1, AGO2, AGO3 and AGO4, but not with AGO5 and AGO9. As an effective suppressor, the amount of AGO1 accumulated in the presence of P25 was dramatically lower than that infiltrated with HcPro, but was restored when treated with a proteasome inhibitor MG132. These findings are consistent with the idea that RNA silencing is an antiviral defence mechanism and that the counter-defence role of P25 is through the degradation of AGO proteins via the proteasome pathway. Further support for this idea is provided by the observation that plants treated with MG132 are less susceptible to PVX and its relative Bamboo mosaic virus.en_US
dc.language.isoen_USzh_TW
dc.relationMolecular Plant Pathologyen_US
dc.relation.ispartofseriesMolecular Plant Pathology, Volume 11, Issue 5, Page(s) 641-649.en_US
dc.relation.urihttp://dx.doi.org/10.1111/j.1364-3703.2010.00634.xen_US
dc.subjecttriple gene blocken_US
dc.subjectto-cell movementen_US
dc.subjectarabidopsis argonaute1en_US
dc.subjectnicotiana-benthamianaen_US
dc.subjectmosaic-virusen_US
dc.subjectrnaen_US
dc.subjectproteinen_US
dc.subjectplantsen_US
dc.subjectdefenseen_US
dc.subjectplasmodesmataen_US
dc.titleThe silencing suppressor P25 of Potato virus X interacts with Argonaute1 and mediates its degradation through the proteasome pathwayen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1111/j.1364-3703.2010.00634.xzh_TW
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:生物科技學研究所
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